食品科学 ›› 2013, Vol. 34 ›› Issue (3): 212-216.

• 生物工程 • 上一篇    下一篇

耐受高体积分数丁醇乳酸菌的筛选及其鉴定

刘洪儒1,曹龙奎2,孙大庆3   

  1. 1. 黑龙江八一农垦大学
    2. 黑龙江八一农垦大学食品学院;黑龙江省农产品加工工程技术研究中心
    3. 黑龙江农产品加工工程中心
  • 收稿日期:2011-10-18 修回日期:2013-01-04 出版日期:2013-02-15 发布日期:2017-12-29
  • 通讯作者: 曹龙奎 E-mail:longkuicao@yahoo.com.cn
  • 基金资助:
    黑龙江省研究生创新科研项目

Screening and Identification of High Butanol Tolerance Lactic Acid Bacteria

  • Received:2011-10-18 Revised:2013-01-04 Online:2013-02-15 Published:2017-12-29

摘要: 从不同样品中分离和筛选在较高丁醇体积分数下生长的菌株,通过不断提高培养基中的丁醇添加量测定菌株的相对生长率,经过初测和复测,选择相对生长率较高的菌株绘制其耐受曲线,分析其能够耐受丁醇的体积分数。对菌株进行常规生理生化鉴定和16S rDNA序列分析,结合菌体形态及菌落特征确立菌株的种属。筛选得到2株耐受丁醇的体积分数均达到了3%的菌株,分别为A37和A50。经鉴定,序列分析与生理生化实验结果是一致的,A37为Enterococcus faecalis,A50为Lactobacillus fermentum。结果表明两株菌具有较高的丁醇耐受性。

Abstract: Micro-organisms was to isolated and screened from different samples which could grow at a high butanol concentration,through repeated the determination of the relative growth rate in the medium what was increased the adding amount of butanol continuously, After repeatedly, Chose strains with higher relatively growth rate and drew the resistance curve, analysis the butanol concentration of strains can tolerate. The strains were identified by physiological and biochemical experiments and 16 S rDNA series analysis, combined with the bacteria and bacterial colonies characteristics, establish the species of the strains.The concentration of butanol that two bacterial strains toleranced were all in 3% (v/v). The 16S rDNA sequences analysis and biochemical Properties test result of two strains was consistent, which revealed that A37 and A50 was Enterococcus faecalis and Lactobacillus fermentum, respectively. Results showed that two bacterial strains has high butanol tolerability, for the next step to the strains of metabolic engineering transformation production butanol laid a foundation.

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