食品科学 ›› 2013, Vol. 34 ›› Issue (1): 268-271.

• 营养卫生 • 上一篇    下一篇

上海市食源性金黄色葡萄球菌分布状况

李自然, 施春雷, 宋明辉, 史贤明*   

  1. 上海交通大学农业与生物学院,中美食品安全联合研究中心,陆伯勋食品安全研究中心
  • 收稿日期:2011-11-14 修回日期:2012-12-17 出版日期:2013-01-15 发布日期:2013-01-07
  • 通讯作者: 史贤明 E-mail:xmshi@sjtu.edu.cn
  • 基金资助:

    国家国际科技合作项目;国家“863”计划项目

Distribution Status of Food-borne Staphylococcus aureus in Shanghai

  • Received:2011-11-14 Revised:2012-12-17 Online:2013-01-15 Published:2013-01-07

摘要: 采集上海市食品样品(生牛乳、生鲜肉、水产品、速冻食品、果蔬、豆制品)505份,按国标GB 4789.10—2010《金黄色葡萄球菌检验》方法进行金黄色葡萄球菌的分离和鉴定,并利用PCR技术进行进一步验证。结果表明:505份食品样品中有117份检出了金黄色葡萄球菌,总检出率为23.2%,其中生鲜肉检出率最高,为32.9%;其次为生牛乳和速冻食品,分别为26.3%和26.7%;其他食品中金黄色葡萄球菌的检出率相对较低。该实验结果基本上反映了上海市各类食品中金黄色葡萄球菌的分布状况,为监控金黄色葡萄球菌引起的食物中毒及追溯污染源提供参考。

关键词: 金黄色葡萄球菌, 分离鉴定, 食品, 多聚酶链式反应

Abstract: In this study, Staphylococcus aureus was isolated and identified from 505 food samples (including raw milk, raw meat, fruits, vegetables, aquatic products, frozen foods and soybean products) collected in Shanghai according to GB4789.10—2010. In addition, PCR was performed for further confirmation. Staphylococcus aureus was detected in 117 food samples, with a total detection rate of 23.2%. The highest detection rate (32.9%) of Staphylococcus aureus in raw meat was found followed by frozen foods (26.7%) and raw milk (26.3%). In addition, the detection rates of Staphylococcus aureus in fruits and vegetables, aquatic products and soy products were lower than 16%. These results revealed the basic distribution status of Staphylococcus aureus in some types of foods in Shanghai, which may provide clues for monitoring Staphylococcus aureus in food processing, controlling food poisoning and tracing pollution sources.

Key words: Staphylococcus aureus, isolation and identification, food, PCR

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