金针菇子实体多糖均一组分FVP60-C2制备及单糖组成研究

食品科学 ›› 2013, Vol. 34 ›› Issue (3): 86-89.

金针菇子实体多糖均一组分FVP60-C2制备及单糖组成研究

杨俊红,张安强,孙培龙

浙江工业大学

收稿日期:2011-12-12 修回日期:2013-01-09 出版日期:2013-02-15 发布日期:2017-12-29
通讯作者: 孙培龙 E-mail:sun_pl@zjut.edu.cn
基金资助:
浙江省食用菌创新团队项目

Preparation and monosaccharide composing analysis of homogeneous polysaccharide FVP60-C2 from the fruiting bodies of Flammulina velutipes

Received:2011-12-12 Revised:2013-01-09 Online:2013-02-15 Published:2017-12-29

摘要/Abstract

摘要： 金针菇子实体经水提醇沉得水溶性粗多糖，再经DEAE-Sepharose Fast Flow离子交换柱和凝胶柱层析纯化，得到均一多糖FVP60-C2；高效液相色谱、紫外全扫描和红外光谱研究其理化性质；样品经三氟乙酸水解，乙酰衍生化后，用单糖标准品作对照，用气相色谱分析其单糖组成。结果表明：FVP60-C2为均一多糖，相对分子质量为1.429 ×104 Da；其是由岩藻糖、木糖、甘露糖、葡萄糖和半乳糖组成的杂多糖，各单糖的摩尔比为0.74∶0.21∶1.36∶1.00∶1.31。

Abstract: The polysaccharide was extracted from fruiting bodies of Flammulina velutipes by hot water extraction, ethanol precipitation. The crude polysaccharide was purified to obtain FVP60-C2 by Ion exchange column chromatography and Gel-filtration column chromatography. The physicochemical properties of FVP60-C2 were investigated by HPLC, UV and IR. Following the hydrolysis and acetylation of FVP60-C2, the monosaccharide composition of FVP60-C2 was analyzed by GC. The results showed a homogeneous polysaccharide FVP60-C2, with molecular weight 1.429×104 Da, was obtained, which is consist of fucose, xylose, mannose, glucose and galactose with the molar ratio of 0.74:0.21:1.36:1.00:1.31.

中图分类号:

TS201.1

杨俊红张安强孙培龙. 金针菇子实体多糖均一组分FVP60-C2制备及单糖组成研究[J]. 食品科学, 2013, 34(3): 86-89.

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