响应面法优化超滤提纯无梗五加果多糖工艺研究

食品科学 ›› 2013, Vol. 34 ›› Issue (2): 67-71.

响应面法优化超滤提纯无梗五加果多糖工艺研究

冯颖

沈阳农业大学食品学院副教授

收稿日期:2011-10-31 修回日期:2012-12-25 出版日期:2013-01-25 发布日期:2013-01-15
通讯作者: 冯颖 E-mail:fywjg@sina.com
基金资助:
辽宁省博士科研启动基金项目

Optimization of Ultrafiltration Technology of Polysaccharide from Acanthopanax sessiliflorus Fruits by Response Surface Methodology

Received:2011-10-31 Revised:2012-12-25 Online:2013-01-25 Published:2013-01-15

摘要/Abstract

摘要： 研究了响应面法超滤提纯无梗五加果多糖工艺。在单因素实验的基础上，利用minitab15软件设计了响应面实验。研究了超滤压力，超滤时间，超滤温度，料液浓度对超滤膜通量的影响并确定最佳工艺参数。结果表明超滤压力0.30 MPa，超滤时间10min，超滤温度40℃，料液浓度1.8mg/mL，预测超滤膜通量达到理论值最高0.077 mL/cm2?min，实际膜通量值为0.070 mL/cm2?min，与预测值基本相符。

Abstract: The ultrafiltration technology of separating polysaccharide from Acanthopanax sessiliforous fruits was studied. On the basis of single factor tests, response surface methodology was designed by minitab software 15. The effect of ultrafiltration pressure, time, temperature and feeding concentration on membrane flux was studied. The results showed that the optimum ultrafiltration conditions were ultrafiltration pressure of 0.30MPa, ultrafiltration time of 10min, ultrafiltration temperature of 40℃ and feeding concentration of 1.8mg/mL.The practical membrane flux was 0.070 mL/cm2?min，close to the predicted membrane flux of 0.077 mL/cm2?min.

中图分类号:

O629.12

冯颖. 响应面法优化超滤提纯无梗五加果多糖工艺研究[J]. 食品科学, 2013, 34(2): 67-71.

参考文献

[1]高凤兰,孙振方,哈永年,等.无梗五加原植物及其生态分布[J].中国中医药科技,1997,4(2):106
[2]淳于家龙,郭丽娜,张长顺.无梗五加化学成分与药理活性的研究进展[J].中国药业, 2002,11(2):73-74
[3]LEE SANG H, JI J, SHIN Kuk H, et al. Sessiline, a new nitrogenous compound from the fruits of Acanthopanax sessiliflorus[J].Planta Medica,2002,68(10):939-941
[4]安琪,杨春娟,宋洋,等.无梗五加果化学成分的研究[J].天然产物研究与开发, 2008,2(5):765-769
[5]LEE SANG H, LEE YEON S, JUNG SANG H, et al. Antitumor and immunostimulating activities of Acanthopanax sessiliflorus fruits[J].Natural Product Sciences, 2003,9(2):112-116
[6]YANG Chunjuan, AN Qi, XIONG Zhili. Triterpenes from Acanthopanax sessiliflorus fruits and their antiplatelet aggregation activities[J].Planta medica,2009,75(6):656-659
[7]冯颖,王建国,孟宪军,等.无梗五加果黄酮类化合物生物活性研究[J].食品研究与开发,2008,29(1):30-33
[8]韩莹,李遇伯,崔兰冲,等.HPLC法同时测定无梗五加果中东莨菪内酯和异嗪皮啶的含量[J].沈阳药科大学学报,2006,23(9):573-576
[9]冯颖, 孟宪军, 王建国, 等.无梗五加果多糖组成及生物活性研究[J].食品科学, 2008,29(4):378-380
[10]李琳,朱杰,傅小琴等.中试超滤系统浓缩分离南瓜多糖[J].食品科学, 2009,30(22):33~34
[11]吴建中,郭开平.采用超滤技术浓缩提纯南瓜多糖[J].食品研究与开发, 2006,28(2):41-43
[12]HU Yaqin, CAO Yang. Applications in separation of polysaccharides by ultrafiltration [J]. Letters in Biotechnology, 2005, 16(2): 228-230.
[13]杨阳,刘承初,贾薇等.灰树花多糖的超滤分离及免疫活性研究[J].食品科学, 2008,28(9):277~278
[14]HOU Xujie, CHEN Wei. Optimization of extraction process of crude polysaccharides from wild edible Bachu mushroom by response surface methodology[J].Carbohydrate Polymers,2008,72(1):67-74
[15]ZHONG Kui, WANG Qiang. Optimization of ultrasonic extraction of polysaccharides from dried longan pulp using response surface methodology[J].Carbohydrate Polymers,2010(80):19-25
[16]CHEN Xiao Ping, WANG Xiang , LI Shuibing, et al. Optimization of ultrasound-assisted extraction of LingZhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells [J].Carbohydrate Polymers,2010(80):944-948
[17]冯颖,陈巧红,孟宪军,等.响应面法优化无梗五加果多糖超声波、微波法提取工艺研究[J].食品科学,2010,31(22):268-272
[18]李芙蓉,吕博.刺五加多糖的微波萃取及含量测定[J].新疆中医药,2003,21(1):11
[19]陈莉,屠康,王海,等.采用响应曲面法对蔡侯红富士苹果热处理条件的优化[J].农业工程学报,2006,22(2):159-163.