食品科学 ›› 2013, Vol. 34 ›› Issue (2): 212-216.

• 工艺技术 • 上一篇    下一篇

不同品种川陈皮中黄酮类物质的含量比较

游元元   

  1. 成都医学院
  • 收稿日期:2011-11-11 修回日期:2012-12-04 出版日期:2013-01-25 发布日期:2013-01-15
  • 通讯作者: 游元元 E-mail:ym633@sina.com
  • 基金资助:
    四川省教育厅科研项目;成都医学院自然科学科研基金项目

Comparison of Flavonoids in Tangerine Peels of Different Varieties from Sichuan

  • Received:2011-11-11 Revised:2012-12-04 Online:2013-01-25 Published:2013-01-15

摘要: 目的 比较不同品种川陈皮中黄酮类物质的含量差异。 方法 采用紫外分光光度法在284 nm处测定吸光度,以橙皮苷计川陈皮中总黄酮的含量。采用高效液相色谱法同时测定各样品中橙皮苷、川陈皮素、红橘素的含量。色谱柱为Phenomenex Luna C18(2) (250 mm×4.6 mm,5 μm),流动相为乙腈-水梯度洗脱,流速为1.0 mL?min-1,检测波长280 nm,柱温25 ℃。 结果 不同品种川陈皮中总黄酮及3种活性黄酮物质含量差异明显。20个川陈皮样品总黄酮变幅为0.64~5.67%,橙皮苷变幅为0.12~1.26%,川陈皮素变幅为0.02~0.61%,红橘素变幅为0.01~0.22%,且不同样品中三种黄酮物质在色谱图总峰面积中所占比例不尽相同。结论 遗传背景、生长环境、栽培方式等因素对川陈皮中黄酮类物质累积有影响,多指标成分更能反映陈皮品质的客观情况。

Abstract: Objective: To compare the contents of flavonoids in tangerine peels of different varieties from Sichuan. Methods: The total flavonoids were determined by ultraviolet spectrophotometry at the wavelengths of 284 nm with hesperidin as a standard,and high performance liquid chromatography was used to analyze the contents of hesperidin, nobiletin and tangeretin simultaneously. The chromatographic separation was performed on a Phenomenex Luna C18(2) column (250 mm×4.6 mm,5 μm) by gradient elution using water-acetonitrile as the mobile phase at a flow rate of 1.0 mL?min-1. The detection wavelength was 280 nm and the column temperature was set at 25 ℃. Results: The results indicated that significan differences in contents of total flavonoids and three bioactive compounds existed in 20 samples from different source. The content ranges of total flavonoids,hesperidin, nobiletin, tangeretin were 0.64-5.67%,0.12-1.26%,0.02-0.61%,0.01-0.22%,respectively. The peak area of three flavonoids in samples had different proportion in total peak area. Conclusion: Flavonoids in tangerine peels were influenced by genetic background, growth environment as well as cultivation method. Multiple active chemical markers provided more quality information of tangerine peels.

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