固相萃取-高效液相法测定蔬菜中磺胺类药物

食品科学 ›› 2013, Vol. 34 ›› Issue (2): 204-208.

固相萃取-高效液相法测定蔬菜中磺胺类药物

刘雪,余晟,韦彩云

信阳师范学院化学化工学院

收稿日期:2011-11-14 修回日期:2012-12-07 出版日期:2013-01-25 发布日期:2013-01-15
通讯作者: 刘雪 E-mail:liuxue1369@126.com
基金资助:
河南省基础与前沿技术研究计划项目

Determination of sulfonamide in vegetable by solid phase extraction- high performance liquid phase chromatography

Received:2011-11-14 Revised:2012-12-07 Online:2013-01-25 Published:2013-01-15

摘要/Abstract

摘要：

以石墨烯为吸附剂制备了固相萃取柱。建立了固相萃取-高效液相色谱法检测6种磺胺类药物的方法。采用紫外检测器进行定性和定量分析，检测波长为270 nm。对包括吸附剂的种类、洗脱剂的种类和用量、样品溶液的pH和样品体积等参数进行了详细优化。在优化条件下，6种磺胺类药物在0.1~100 μg/L浓度范围内与峰面积呈良好的线性关系，相关系数为0.990~0.997。信噪比为3时，检出限为6.3~21.1 ng/L。方法的精密度为2.12~4.75%。将该方法用于蔬菜样品分析，回收率为78.5%~103.2%。

Abstract:

A solid extraction column was prepared with graphene as adsorbent. Analytical method for the determination of six sulfonamides was developed based on graphene solid-phase extraction (SPE) coupled with high-performance liquid-phase chromatography. Ultraviolet detection was used for qualitative and quantitative analysis at 270 nm. Factors that may influence the extraction efficiency including the kind of adsorbents, the kind and volume of eluent, pH of sample solutions and sample volume were optimized in detail. Under the optimized conditions, there was good linearity in the concentration range of 0.1-100 μg/L with the correlation coefficients of 0.990-0.997. Limits of detection were in the range of 6.3-21.1 ng/L (S/N = 3). The precisions of proposed method were in the range of 2.12-4.75%. The method was applied to the analysis of vegetable samples with recoveries of 78.5% -103.2%.

中图分类号:

O657.72

刘雪余晟韦彩云. 固相萃取-高效液相法测定蔬菜中磺胺类药物[J]. 食品科学, 2013, 34(2): 204-208.

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