Abstract: The Study of Mechanism of 6-Hydroxykaempferol-3-O-β-glucoside for Inhibiting Oxidative Damage of Deoxyribose WANG Yuan-kai1，ZHI peng2，ZHANG Li-wei1,* (1. Institute of Molecular Science, Shanxi University, Taiyuan 030006, China； 2. School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072, China) Abstract：Objective：To study the ability of 6-hydroxykaempferol-3-O-β-glucoside(6-HK-3-O-G) for inhibiting Fenton reaction and scavenging hydroxyl radical , and to explore the mechanism of inhibiting the Fenton reaction . Methods: the detection model of deoxyribose oxidative damage and scavenging DPPH?was used . Results 6-HK-3-O -G not only has strong ability of scavenging hydroxyl radical, but also can protect 2-deoxyribose oxidative damage which caused by the Fenton reaction. There are two reasons for 6-HK-3-O-G protecting deoxyribose oxidative damage，one of the reasons is the strong ability of 6-HK-3-O-G scavenging hydroxyl radical, and more important reasons is the chelation of 6-HK-3-O-G with Fe2+ , which can block the Fenton reaction. Conclusions: 6-HK-3-O-G do has strong anti-oxidation activity, and the chelation of 6-HK-3-O-G with Fe2+ may play a vital role for its inhibiting Fenton chemistry under physiological conditions.