姬菇多糖对酒精所致小鼠急性肝损伤的保护作用

食品科学 ›› 2012, Vol. 33 ›› Issue (23): 323-326.

姬菇多糖对酒精所致小鼠急性肝损伤的保护作用

周斌1，陶明煊1,*，程光宇2，郭永月1

1.南京师范大学金陵女子学院 2.南京师范大学生命科学学院

收稿日期:2011-10-21 修回日期:2012-11-20 出版日期:2012-12-15 发布日期:2012-12-12
通讯作者: 陶明煊 E-mail:45017@njnu.edu.cn
基金资助:
江苏省高校自然科学基础研究项目;江苏省普通高校研究生科研创新计划项目

Protective Effect of Polysaccharides from Pleurotus cornucopiae against Alcohol-Induced Acute Liver Injury in Mice

Received:2011-10-21 Revised:2012-11-20 Online:2012-12-15 Published:2012-12-12

摘要/Abstract

摘要： 目的：研究热水浸提法从姬菇中提取姬菇多糖(PCNP)对酒精所致小鼠急性肝损伤的保护作用。方法：小鼠被随机分为空白对照组、模型组、阳性对照组(150mg/(kg·d))、多糖各剂量组(100、200、400mg/(kg·d))，连续灌胃30d后，除空白对照组外，给予50%体积分数的乙醇溶液建立小鼠急性肝损伤模型。12h后，小鼠脱臼处死后取血液和肝脏测定各项抗氧化指标，并观察肝组织病理学变化。结果：与模型组相比，多糖各剂量组能降低血清谷草转氨酶(AST)、谷丙转氨酶(ALT)活性和肝脏丙二醛(MDA)含量，提高肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性、谷胱甘肽(GSH)含量。结论：PCNP能明显改善肝组织病理损伤程度，对酒精所致小鼠急性肝损伤具有明显保护作用。

关键词: 姬菇多糖, 急性肝损伤, 抗氧化, 血清, 酒精

Abstract: The present study investigated the protective effect of polysaccharides in hot water extract from Pleurotus cornucopiae (PCNP) on alcohol-induced acute liver injury in mice. Mice were randomly divided into blank control, alcohol-induced liver injury model, positive control and PCNP treatment (at doses of 100, 200 mg/(kg·d) and 400 mg/(kg·d) groups. After 30 consecutive days of intragastric administration, all the mice except for blank control group were given by gavage 50% alcohol at a dose of 12 mL/kg to induce acute liver injury. At 12 h after alcohol administration, all the mice in each group were killed by cervical dislocation and blood and liver were collected for the measurement of antioxidant indicators and pathological observation of liver tissue. Compared with the model group, PCNP administration resulted in a decrease in MDA content in the liver and the activities of AST, ALT in the serum, and an increase in the activities of SOD and CAT and GSH content in the liver of mice. In conclusion, PCNP can obviously improve pathological liver injury and protect against acute alcohol-induced liver injury in mice.

Key words: Pleurotus cornucopiae polysaccharides, acute liver injury, antioxidant status, serum, alcohol

中图分类号:

TS201.4

周?斌1，陶明煊1,*，程光宇2，郭永月1. 姬菇多糖对酒精所致小鼠急性肝损伤的保护作用[J]. 食品科学, 2012, 33(23): 323-326.

参考文献

[1] 鞠辉,魏良洲. 酒精性肝病的流行病学现状[J].肝脏,2006,11(1):57-58.
[2] 王波. 彩色图解姬菇、肺形侧耳栽培新技术[M].成都:四川科学出版社,2003:1-2.
[3] 姜自彬,曹锦花,魏学彦. 糙皮侧耳真菌的研究进展[J].中草药,1999,30(1):72-75.
[4] ELUHAN S, RANOGAJEC A. Composition and non-volatile components of croatian wild edible mushrooms. [J]. Food Chemistry, 2011, 124(3):1076-1082.
[5] ELMASTA M, ISILDAK O, TURKEKUL I, et al. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms [J]. Journal of Food Composition and Analysis.2007,20(3):337–345.
[6] WASSER, S P. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides[J]. Microbiol Biotechnol,2002,60(3):258–274.
[7] 王峰, 陶明煊, 程光宇,等. 4种食用菌提取物自由基清除作用及降血糖作用的研究[J]. 食品科学, 2009, 30(21):343-347.
[8] ELBOHI K , SABIK L, MUZANDU K, et al. Antigenotoxic effect of Pleurotus cornucopiae extracts on the mutagenesis of Salmonella typhimurium TA98 elicited by benzo pyrene and oxidative DNA lesions in V79 hamster lung cells[J]. Japanese Journal of Veterinary Research, 2005,52(4):163-172.
[9] KHLOODM E B, YOSHIHARU H, KAAMPWE M, et al. Protective effect of Pleurotus cornucopiae mushroom extract on carbon tetrachloride-induced hepatotoxicity [J].Japanese Journal of Veterinary Research ,2009 ,57(2):109-118.
[10] STEWERT R C, BEWLEY J D. Lipid peroxidation associated with accelerated aging of soybean axes[J]. Plant Physiol, 1980, 65 (2):245-248.
[11] CHIANG M C, CHOU C C. Expression of superoxide dismutase, catalase and thermostable direct hemolysin by, and growth in the presence of various nitrogen and carbon sources of heat-shocked and ethanol-shocked Vibrio parahaemolyticus[J]. International Journal of Food Microbiology.2008,121(3): 268-274.
[12] JAYAKUMAR T, SAKTHIVEL M, THOMAS P A, et al. Pleurotus ostreatus, an oyster mushroom, decreases the oxidative stress induced by carbon tetrachloride in rat kidneys, heart and brain[J]. Chemico-Biological Interactons, 2008, 176(2-3):108-120.
[13] 中华人民共和国卫生部. 保健食品检验与评价技术规范(2003年版)[S].2003:133-139.
[14] 汤宜朗. 酒精戒断综合征:机制、表现及处理[J].临床精神医学杂志, 1996, 6(4):219-222.
[15] 杜明. 桑黄多糖的提取及其对小鼠急性肝损伤的保护作用研究[D]. 杨凌: 西北农林科技大学, 2010.
[16] MISHRA A, PAUL S, SWARNAKAR S. Downregulation of matrix metalloproteinase-9 by melatonin during prevention of alcohol-induced liver injury in mice [J]. Biochimie,2011 ,93(5):854-866.
[17] DAI T, WU Y, LENG A S, A YAN, et al.RXRα-regulated liver SAMe and GSH levels influence susceptibility to alcohol-induced hepatotoxicity[J].Experimental and Molecular Pathology, 2003,75(3):194–200.
[18] GOVINDER J S F, PRAHLAD K S.Beneficial Effects of S-Adenosyl-L-Methionine on Aminolevulinic Acid Dehydratase, Glutathione, and Lipid Peroxidation During Acute Lead–Ethanol Administration in Mice. [J].Alcohol , 1999 ,18(2) 103–108.
[19] BALASUBRAMANIYAN V, KALAIVANI SAILAJA J, NALINI N.Role of leptin on alcohol-induced oxidative stress in swiss mice[J]. Pharmacological Research,2003, 47 (3):211–216.
[20] 刘洁,李文香, 王文亮,等. 多糖空间结构与生物活性相关性研究进展[J].农业机械, 2011, 06(17):153-155.
[21] 欧阳天贽,李小定,荣建华. 真菌多糖抗肿瘤及免疫调节作用研究进展[J] .天然产物研究与开发, 2006, 18:524-528.
[22] KIM S W, HWANG H J, BAEK Y M, et al. Proteomic and transcriptomic analysis for streptozotocin-induced diabetic rat pancreas in response to fungal polysaccharide treatments[J].Proteomics.2008, 8(11):2344-2361.
[23] WIJESEKARAA I, PANGESTUTI R, KIM S K, Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae. [J].Carbohydrate Polymers,2011 ,1(11):14-21.
[24] CHOI E Y , HWANG H J, KIM I H, et al.Protective effects of a polysaccharide from Hizikia fusiformis against ethanol toxicity in rats [J].Food and Chemical Toxicology,2009 ,47(1):134-139.
[25] NADA S A, OMARA E A, ZAHRAN H G..Mushroom insoluble polysaccharides prevent carbon tetrachloride-induced hepatotoxicity in rat[J].Food and Chemical Toxicology,2010,48(11):3184-3188.