原花青素长期干预对Ⅱ型糖尿病氧化应激水平的影响

doi:10.7506/spkx1002-6630-201307055

食品科学 ›› 2013, Vol. 34 ›› Issue (7): 262-265.doi: 10.7506/spkx1002-6630-201307055

原花青素长期干预对Ⅱ型糖尿病氧化应激水平的影响

姜燕飞，张召锋，鲍雷，井路路，丁叶，戴小倩，马小陶，李宇杰，蔡夏夏，李勇*

北京大学公共卫生学院营养与食品卫生学系，北京 100191

收稿日期:2011-12-05 修回日期:2013-03-03 出版日期:2013-04-15 发布日期:2013-03-20
通讯作者: 李勇 E-mail:liyong@bjmu.edu.cn
基金资助:
国家自然科学基金项目

Effect of Long-Term Intervention with Proanthocyanidin on Oxidative Stress in Type Ⅱ Diabetes

JIANG Yan-fei，ZHANG Zhao-feng，BAO Lei，JING Lu-lu，DING Ye，DAI Xiao-qian，MA Xiao-tao，LI Yu-jie，CAI Xia-xia，LI Yong*

Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China

Received:2011-12-05 Revised:2013-03-03 Online:2013-04-15 Published:2013-03-20
Contact: LI Yong E-mail:liyong@bjmu.edu.cn

摘要/Abstract

摘要： 目的：观察Ⅱ型糖尿病大鼠经长期喂养后各组织氧化应激水平的改变情况及葡萄籽原花青素提取物(GSPE)长期干预对其的保护作用。方法：将成功建立Ⅱ型糖尿病模型的48只SD大鼠随机分为4组：GSPE低、中、高剂量干预组(分别灌胃125、250、500mg/(kg·d))及糖尿病模型(DM)组；另外12只基础饲料饲养的大鼠作为正常对照组。DM组和正常对照组灌胃等量蒸馏水。连续喂养24周后，取血和脏器，检测各组织的氧化应激指标(超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA))。结果：与正常对照组相比，DM组大鼠血清和肝脏组织中SOD的活性水平显著下降(P＜0.05)，肝脏和肌肉组织中GSH-Px的活性水平亦显著下降(P＜0.05)，脑组织中MDA含量明显增高(P＜0.05)；与DM组相比，GSPE血清、肝脏和脾脏组织中SOD的活性水平显著增加(P＜0.05)，血清、肝脏、肌肉和脾脏中GSH-Px的活性水平亦显著增加(P＜0.05)，脾、脑中MDA含量显著下降(P＜0.05)。结论：Ⅱ型糖尿病大鼠不同组织的氧化损伤程度不同，GSPE长期干预能够减轻各组织的氧化损伤。

关键词: 葡萄籽原花青素提取物, Ⅱ型糖尿病, 长期喂养, 超氧化物歧化酶, 谷胱甘肽过氧化物酶, 丙二醛

Abstract: Objective: To observe the oxidative stress in Type Ⅱ diabetic rats after long-term feeding of grape seed proanthocyanidin extract (GSPE) and the effect of GSPE intervention on oxidative stress. Methods: Forty-eight Type Ⅱ diabetic rats that were successfully induced were randomly divided into 4 groups: three GSPE-treated groups and one diabetic model group. Twelve rats fed on normal forage were served as normal control group. GSPE-treated groups were administered with GSPE by gavage at doses of 125, 250, 500 mg/(kg·d). Diabetic model group and normal control group were administered with deionized water also by gavage. After 24 weeks, all rats were killed and blood and organs were acquired. Oxidative indicators (SOD, GSH-Px and MDA) of all tissues were detected. Results: Compared with the normal control group, SOD activity in the serum and liver tissue of the diabetic model group was significantly decreased, so was the GSH-Px activity in liver and muscle tissue, but the amount of MDA in the brain of the diabetic model group was increased; compared with the diabetic model group, SOD activity in the serum, liver and spleen and GSH-Px activity in the serum, liver, muscle and spleen from the intervention groups was significantly increased (P ＜ 0.05). Conclusion: The level of oxidative damage in different tissues from Type Ⅱ diabetic rats is different. Long-term intervention with GSPE can reduce oxidative damage to tissues.

Key words: grape seed proanthocyanidin extract, Type Ⅱ diabetes, long-term feeding, superoxide dismutase, glutathione peroxidase, malonaldehyde

中图分类号:

151.2

姜燕飞，张召锋，鲍雷，井路路，丁叶，戴小倩，马小陶，李宇杰，蔡夏夏，李勇*. 原花青素长期干预对Ⅱ型糖尿病氧化应激水平的影响[J]. 食品科学, 2013, 34(7): 262-265.

JIANG Yan-fei，ZHANG Zhao-feng，BAO Lei，JING Lu-lu，DING Ye，DAI Xiao-qian，MA Xiao-tao，LI Yu-jie，CAI Xia-xia，LI Yong*. Effect of Long-Term Intervention with Proanthocyanidin on Oxidative Stress in Type Ⅱ Diabetes[J]. FOOD SCIENCE, 2013, 34(7): 262-265.

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原花青素长期干预对Ⅱ型糖尿病氧化应激水平的影响

Effect of Long-Term Intervention with Proanthocyanidin on Oxidative Stress in Type Ⅱ Diabetes

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