大孔吸附树脂分离纯化五倍子鞣质

食品科学 ›› 2012, Vol. 33 ›› Issue (20): 74-79.

大孔吸附树脂分离纯化五倍子鞣质

高燕1,3，李勤1,2，盛达成1,2，肖文军1,2,3,*，刘仲华1,2,3，张盛1,2，傅冬和1,2

1.湖南农业大学国家植物功能成分工程技术研究中心 2.湖南农业大学园艺园林学院植物资源工程系 3.湖南农业大学园艺园林学院茶学系

收稿日期:2011-08-01 修回日期:2012-09-18 出版日期:2012-10-25 发布日期:2012-11-09
通讯作者: 肖文军 E-mail:xiaowenjun88@sina.com;xiaowenjungong@163.com
基金资助:
“十二五”国家支撑计划资助项目

Separation and Purification of Tannin from Galla chinensis by Macroporous Resin Adsorption

Received:2011-08-01 Revised:2012-09-18 Online:2012-10-25 Published:2012-11-09

摘要/Abstract

摘要： 以五倍子鞣质粗提物为原料，通过静态吸附与解吸实验比较NKA-2、NKA-9、HPD100、AB-8、D101、D301、聚酰胺及732八种吸附树脂对五倍子鞣质的吸附与解吸性能，筛选得到最优树脂NKA-2。然后通过动态吸附与解吸的单因素试验和正交试验，优化筛选NKA-2大孔吸附树脂分离纯化五倍子鞣质的工艺技术参数。结果表明，当主要考虑鞣质得率时，最优工艺参数为上样质量浓度5mg/mL、上样流速1BV/h、上样体积6BV、洗脱剂乙醇体积分数80%、洗脱流速1BV/h、洗脱体积4BV，其鞣质得率可达85.37%，纯度可达62.99%；当主要考虑鞣质纯度时，最优参数为上样质量浓度6mg/mL、上样流速2BV/h、上样体积5BV、洗脱剂乙醇体积分数80%、洗脱流速3BV/h、洗脱体积4BV，其鞣质纯度可达76.97%，得率达67.78%。

关键词: 五倍子, 鞣质, 大孔吸附树脂, 分离纯化

Abstract: NKA-2 macoporous adsorption resin was the best resin for the separation and purification of crude tannin extract from Galla chinensis as demonstrated by a comparison of the effectiveness of 8 types of macoporous adsorption resin in static adsorption and desorption of tannin. One-factor-at-a-time and orthogonal array design were used to optimize dynamic adsorption and desorption parameters for the purification of the crude tannin extract. The optimal process parameters for maximum tannin yield were determined as follows: 6 BV of 5 mg/mL sample was loaded onto the column at a flow rate of 1 BV/h and after complete adsorption, the column was then eluted with 4 BV of 80% ethanol solution at a flow rate 1 BV/h, yielding a tannin yield of 85.37% and a tannin purity of 62.99%. However, the optimal process parameters for maximum tannin purity were determined as follows: 5 BV of 6 mg/mL sample was loaded onto the column at a flow rate of 2 BV/h and after complete adsorption, the column was then eluted with 4 BV of 80% ethanol solution at a flow rate 3 BV/h, yielding a tannin yield of 76.97% and a tannin purity of 67.78%.

Key words: Galla chinensis, tannin, macroporous resin, separation and purification

中图分类号:

高?燕1,3，李?勤1,2，盛达成1,2，肖文军1,2,3,*，刘仲华1,2,3，张?盛1,2，傅冬和1,2. 大孔吸附树脂分离纯化五倍子鞣质[J]. 食品科学, 2012, 33(20): 74-79.

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