食品科学 ›› 2012, Vol. 33 ›› Issue (23): 228-231.

• 生物工程 • 上一篇    下一篇

从天山花楸中分离筛选纤维素酶产生菌及其酶学性质研究

付建红,任晓源,范秋霜,谢连珍,谢红桃   

  1. 新疆师范大学生命科学学院
  • 收稿日期:2011-10-13 修回日期:2012-10-25 出版日期:2012-12-15 发布日期:2012-12-12
  • 通讯作者: 付建红 E-mail:fjh_719@163.com
  • 基金资助:

    新疆维吾尔自治区高校科研计划青年教师科研启动基金项目;新疆师范大学博士科研启动基金项目;新疆师范大学校级重点实验室开放基金项目

Screening of Cellulase-Producing Bacterium from Sorbus tianshanica and Enzymatic Characteristics

  • Received:2011-10-13 Revised:2012-10-25 Online:2012-12-15 Published:2012-12-12
  • Contact: Jian-Hong FU E-mail:fjh_719@163.com

摘要: 从天山花楸茎、叶和果实中分离到53株内生细菌,利用刚果红初筛平板获得透明圈较大的4株菌,在此基础上进行摇瓶复筛,得到一株产纤维素酶活力较高的菌株(S11)。该菌发酵培养12h,产羧甲基纤维素酶(CMCase)酶活力最高达到39.47U/mL。该酶最适反应pH值为5.0,在pH 5.0~6.0范围内较稳定;最适反应温度为50℃,在50℃以下酶的热稳定性较好,60~70℃几乎完全失活;各种供试金属离子与化学试剂对酶活性都有不同程度的抑制作用,其中Ca2+、SDS和EDTA的抑制作用最强。

关键词: 天山花楸, 纤维素酶产生菌, 酶活性, 酶学性质

Abstract: Fifty-three endophytic bacteria were isolated from Sorbus tianshanica Rupr. stems, leaves and fruits. Four colonies were surrounded by big transparent zones in congo red agar medium. A strain (S11) with higher cellulase activity was isolated and cultured in shake flasks for secondary screening. Maximum carboxymethyl cellulase (CMCase) activity was found to be 39.47 U/mL after 12 h culture. The optimal reaction pH for crude CMCase solution was 5.0, and it revealed high stability at pH 5.0–6.0. The optimal reaction temperature was 50 ℃, and its activity remained stable below 50 ℃ but was almost completely lost at 60–70 ℃. The CMCase activity was inhibited by all tested metal ions and compounds, and dramatically inhibited by Ca2+, SDS and EDTA.

Key words: Sorbus tianshanica Rupr., cellulase-producing bacterium, cellulase activity, enzymatic characteristics

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