茶叶儿茶素对肠道微生态的调节作用

食品科学 ›› 2013, Vol. 34 ›› Issue (5): 232-237.

茶叶儿茶素对肠道微生态的调节作用

张鑫1，马丽苹2，张芸1，高远1，胡冰1，曾晓雄1,*

1.南京农业大学食品科技学院，江苏南京 210095；2.河南科技大学食品与生物工程学院，河南洛阳 471003

收稿日期:2011-11-01 修回日期:2013-01-15 出版日期:2013-03-15 发布日期:2013-04-16
通讯作者: 曾晓雄 E-mail:zengxx2002@yahoo.com.cn
基金资助:
江苏省科技支撑项目;江苏高校优势学科建设工程资助项目

Regulatory Effect of Tea Catechins on Intestinal Microecology

ZHANG Xin1，MA Li-ping2，ZHANG Yun1，GAO Yuan1，HU Bing1，ZENG Xiao-xiong1,*

1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China； 2. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China

Received:2011-11-01 Revised:2013-01-15 Online:2013-03-15 Published:2013-04-16
Contact: Xiaoxiong Zeng E-mail:zengxx2002@yahoo.com.cn

摘要/Abstract

摘要： 以中国乌龙茶为材料，通过热水浸提与柱层析分离纯化，制备得到纯度30% EGCG3’’Me、50%EGCG3’’Me、90% EGCG3’’Me和90% EGCG的茶叶儿茶素样品。采用体外厌氧粪样混合培养与荧光原位杂交技术，评价各茶叶儿茶素对于肠道微生物菌群生长的影响。结果显示：甲基化儿茶素能促进肠道有益细菌(双歧杆菌和乳酸菌)的增殖，抑制有害细菌(梭状菌和拟杆菌)的增殖，但对肠道总菌群数量的影响不大。90% EGCG3’’Me样品的益生指数(PI)最高(2.68)，50% EGCG3’’Me、30% EGCG3’’Me和90% EGCG的样品PI值依次为2.25、2.14和1.86，而对照组的PI值为0.12。以上结果表明茶叶儿茶素特别是甲基化儿茶素具有一定的调节肠道微生态的能力，甲基化儿茶素的纯度越高，发挥的作用越为明显。

关键词: 茶叶, 甲基化儿茶素, 荧光原位杂交, 肠道微生态

Abstract: Tea catechins were extracted by hot water from Chinese Oolong tea, and purified by column chromatography to obtain the samples containing 30% EGCG3’’Me, 50% EGCG3’’Me, 90% EGCG3’’Me and 90% EGCG, respectively. The effects of these prepared samples on intestinal microflora were evaluated by in vitro fermentation of human fecal bacteria and fluorescent in situ hybridization. Results showed that methylated catechins exhibited a proliferative effect on probotics such as Bifidobacterium spp. and Lactobacillus/Enterococcus spp., and an inhibitory effect on harmful bacteria such as Bacteroids-Prevotella spp. and Clostridium histolyticum. However, no significant difference in the number of total bacteria between treatment and control groups was observed. In addition, 90% EGCG3’’Me had the highest probiotic index (PI, 2.68), followed by 50% EGCG3’’Me (2.25), 30% EGCG3’’Me (2.14) and 90% EGCG (1.86), which were much higher than that of the control (0.12). These results suggested that methylated catechins might have certain regulatory effect on intestinal environment by modulating intestinal microecology.

Key words: tea, methylated tea catechins, FISH, intestinal microecology

中图分类号:

张鑫1，马丽苹2，张芸1，高远1，胡冰1，曾晓雄1,*. 茶叶儿茶素对肠道微生态的调节作用[J]. 食品科学, 2013, 34(5): 232-237.

ZHANG Xin1，MA Li-ping2，ZHANG Yun1，GAO Yuan1，HU Bing1，ZENG Xiao-xiong1,*. Regulatory Effect of Tea Catechins on Intestinal Microecology[J]. FOOD SCIENCE, 2013, 34(5): 232-237.

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