应用焦磷酸测序技术快速检测食品中沙门氏菌

食品科学 ›› 2013, Vol. 34 ›› Issue (2): 182-186.

应用焦磷酸测序技术快速检测食品中沙门氏菌

赵新

天津农科院中心实验室

收稿日期:2011-08-25 修回日期:2012-12-04 出版日期:2013-01-25 发布日期:2013-01-15
通讯作者: 赵新 E-mail:zhaoxin2008999@126.com

Application on Rapid Detection of Salmonellla spp in food by pyrosequencing technology

Received:2011-08-25 Revised:2012-12-04 Online:2013-01-25 Published:2013-01-15

摘要/Abstract

摘要：

以沙门氏菌invA毒力靶基因为主要研究对象，采取焦磷酸测序技术，通过对沙门氏菌invA毒力靶基因特异性序列分析，设计出适合焦磷酸测序的特异性扩增引物和测序引物，同时对焦磷酸测序反应条件进行了优化，建立一种利用焦磷酸测序技术检测和鉴定沙门氏菌的方法。结果显示，此方法以传统国标法的前增菌为前提，而省去了后期生化验证的繁琐过程，将检测时间由常规检测的4～7天缩短至20小时，并且其准确性与传统生化验证完全一致。所建立的针对沙门氏菌的焦磷酸测序检测方法具有高效性、精准及操作简便等特点，适用于食品中沙门氏菌的快速检测。

Abstract:

To develop a technique for detecting and identifying Salmonellla spp by using pyrosequencing technology. According to virulence-specific target gene sequence analysis, this study designed specific PCR primers and sequencing primers respectively for pyrosequencing, and pyrosequencing reaction conditions were optimized. The results show that this method is the traditional national standard method of enrichment as a precondition before, while eliminating the tedious process of post-biochemical validation, greatly reducing the detection time from 4 or 7 days to 16 hours and accuracy consistent with the traditional biochemical validation. The study established Pyrosequencing detection method is effective, fast, accurate and simple operation for fast food-borne pathogen detection and identification of precise opened up broad prospects.

中图分类号:

Q781

赵新. 应用焦磷酸测序技术快速检测食品中沙门氏菌[J]. 食品科学, 2013, 34(2): 182-186.

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