食品科学 ›› 2012, Vol. 33 ›› Issue (16): 175-178.

• 分析检测 • 上一篇    下一篇

北五味子种子和果肉中活性成分比较

李立顺,时维静*,马迅兵   

  1. 安徽科技学院食品药品学院
  • 收稿日期:2011-07-29 修回日期:2012-07-08 出版日期:2012-08-25 发布日期:2012-09-07
  • 通讯作者: 时维静 E-mail:shiwj80@yahoo.com.cn
  • 基金资助:

    安徽省科技厅科技攻关重点项目资助;安徽科技学院自然科学研究计划项目

Comparative Analysis of Bioactive Component in Seeds and Flesh of Schisandrae chinensis Fructus by Thin Layer Chromatography

  • Received:2011-07-29 Revised:2012-07-08 Online:2012-08-25 Published:2012-09-07
  • Contact: SHI Wei-Jing E-mail:shiwj80@yahoo.com.cn

摘要: 目的:比较北五味子种子和果肉中活性成分的差异。方法:采用薄层色谱法比较北五味子种子、果肉和果实中木脂素成分含量;采用苯酚-硫酸法测定种子、果肉和果实及果肉不同组分糖的含量;薄层色谱法分析三氟乙酸水解后,果肉不同组分单糖组成。结果:北五味子木脂素类主要在种子中;北五味子在果肉和种子中的总糖含量分别为169.34、5.366mg/g;果肉中各组分糖含量分别为醇溶组分117.06mg/g、醇沉组分75.67mg/g、多糖组分12.37mg/g。三氟乙酸水解后薄层分析,醇溶组分主要由葡萄糖组成,多糖组分主要由葡萄糖、半乳糖组成。结论:北五味子种子和果肉中活性成分存在明显差异。

关键词: 北五味子, 种子, 果肉, 木脂素, 多糖

Abstract: Objective: To comparatively analyze bioactive components in seeds and flesh of Schisandrae chinensis Fructus. Methods: The contents of lignan in seeds, flesh and whole fruit of Schisandrae chinensis Fructus were determined by high performance thin layer chromatography (HPTLC). Sulfuric acid-phenol method was used to quantify sugar. After trifluoroacetic acid hydrolysis, the monosaccharide composition of the flesh was analyzed by HPTLC. Results: Lignans in Schisandrae chinensis Fructus were mainly present in the seeds, and total sugar content was 169.34 mg/g in the flesh and 5.366 mg/g in the seeds. The alcohol-soluble, alcohol-insoluble and polysaccharide components were 117.06, 75.67 mg/g and 12.37 mg/g in the fresh, respectively. The alcohol-soluble fraction was mainly composed of glucose. The polysaccharide fraction was mainly composed of glucose and galactose. Conclusion: Bioactive components reveal a significant difference between seeds and flesh of Schisandrae chinensis Fructus. 

Key words: Schisandrae chinensis Fructus, seeds, flesh, lignanoid, polysaccharides

中图分类号: