响应曲面法优化纤维素酶酶解提取工艺

食品科学 ›› 2013, Vol. 34 ›› Issue (4): 75-79.

响应曲面法优化纤维素酶酶解提取工艺

钱时权

蚌埠学院

收稿日期:2011-11-17 修回日期:2013-01-15 出版日期:2013-02-25 发布日期:2013-01-29
通讯作者: 钱时权 E-mail:qianshq@163.com
基金资助:
安徽省高等学校省级食品科学与工程特色专业建设点;安徽省食品科学与工程教学团队项目;蚌埠学院自然科学研究项目;蚌埠学院优秀人才资助项目

Optimization of cellulase enzymolysis on extraction by response surface methodolog

Shi-Quan QIAN

Received:2011-11-17 Revised:2013-01-15 Online:2013-02-25 Published:2013-01-29
Contact: Shi-Quan QIAN E-mail:qianshq@163.com

摘要/Abstract

摘要： 为优化纤维素酶酶解提取白藜芦醇工艺，在单因素试验的基础上进行响应面试验，选择酶添加量、酶解时间和酶解温度为自变量，以白藜芦醇含量为响应值。结果表明：3个因素对白藜芦醇提取效果影响的主次顺序依次为酶添加量＞酶解时间＞酶解温度，酶添加量为极显著影响因素，酶解时间和酶解温度影响不显著。确定纤维素酶酶解处理山葡萄渣白藜芦醇提取工艺的最佳条件为酶添加量1.30%、酶解时间1.48h、酶解温度55.49℃，在此条件下提取得到的白藜芦醇含量为0.746mg/g，是优化前提取得到的白藜芦醇含量的1.15倍。

关键词: 白藜芦醇, 纤维素酶酶解, 响应曲面分析法

Abstract: To discuss the impact of cellulase on extraction of resveratrol，response surface methodology(RSM) was investigated with the content of resveratrol as the response value and cellulase dosage, enzymolysis time and enzymolysis temperature as indepengdent variables, on basis of single factor experimengts,.The results showed that cellulase dosage had a extremely significant effect on the yield of resveratrol, but enzymolysis time and enzymolysis temperature had no significant effect. The effects order of three factors on content of resveratrol were as follows: cellulase dosage first, enzymolysis time second, and then enzymolysis temperature. The optimum enzymolysis conditions were dosage of cellulase 1.30%, time of enzymolysis 1.48h and temperature of enzymolysis 55.49℃. Under optimal conditions, the content of resveratrol was finally determined to be 0.746 mg.g-1, which was 1.15 times of that of non-optimization.

中图分类号:

TS255
S663

钱时权. 响应曲面法优化纤维素酶酶解提取工艺[J]. 食品科学, 2013, 34(4): 75-79.

Shi-Quan QIAN. Optimization of cellulase enzymolysis on extraction by response surface methodolog[J]. FOOD SCIENCE, 2013, 34(4): 75-79.

参考文献

[1]韩晶晶，刘炜，毕玉平．白藜芦醇的研究进展[J]．生物工程学报，2008，24(11)：1851-1859．
[2]张初署，杨庆利，潘丽娟，等．白藜芦醇的提取及分离纯化研究进展[J]．食品工业科技，2008，29(8)：289-291．
[3] Langcake P, Pryce R. The production of resveratrol by Vitis vinifera and other members of the vitaccae as a response to infection or injury[J]. Physio Plant Path, 1976, 9(11):77-86.
[4]Jeandet P, Bessis R, Maume BF, et a. Effect of enological practices on the resveratrol isomer content of wine[J]. J Agric Food Chem, 1995, 43(2):316-3l9.
[5]Kimura Y, Okuda H. ResveratroI isolated from polygonum cuspidatum root prevents tumor growth and metastasis to Iung and tumor-induced neovascularization in Lewis lung carcinoma-bearing mice[J].J Nutr, 2001, 131:1844-1849.
[6]Jang M, Cai L, Udeani GO, et a1. Cancer chemopreventive activity of resveratrol，a natural product derived from grapes[J].Science,1997,275:2l8-220
[7]Opipari A W J、Resveratrol-induced autophagocytosis in ovarian cancer cells[J]. Cancer Res，2004，64(2)：696-703．
[8] Aggarwal B，Bhardwaj A. Role of resveratrol in prevention and therapy of cancer: preclinical and clinical studies[J].Anticancer Res, 2004, 24(5):2783-2840.
[9]Alkhalaf M. Resveratrol-induced growth inhibition in MDA-MB-231 breast cancer cells is associated with mitogen-activated protein kinase signaling and protein translation[J].Eur J Cancer Prev, 2007, 16(4):334-341.
[10] Levi F, Pasche C. Resveratrol and breast cancer risk[J].Eur J Cancer Prev,2005,14(2):l39-142.
[11] Beata O, Barbara W. Resveratrol, a phenolic antioxidant with effects on blood platelet functions[J]. Platelets, 2005, 16(5):251-260.
[12]张干伟，孙素玲，汤坚．花生根中白藜芦醇的醇提工艺及清除自由基研究[J]．食品科技，2008，33(2)：153-155．
[13] Guiso M, Marra C. A new efficient resveratrol synthesis[J].Tetrahedron Lett,2002, 43(4):597-598.
[14]Pascnal and Marti M C, Salvador A, Chafer A, et al. Supercritical fluid extraction of resveratrol from grape skin of vivis inifera and determination by HPLC[J].Talanta, 2001, 54(4):735-740.
[15] Eder Reinhard, Wendelin Silvia, Vrhovsek Ursa. Resveratrol contents of grapes and red wines in dependency on vintage year and harvest date[J]. Mitteilungen Klostemeuburg, 2001, 51(2-3):64-78.
[16] 李延华，王伟君，张兰威，等．白藜芦醇的研究现状及应用前景[J].中国酿造，2008,184(7):10-12.
[17] 陈秀霞，陈由强，陈如凯．白藜芦醇研究进展[J]．福建林业科技，2003，30(4)：:84-88．
[18]李胜华，伍贤进．超声波法提取虎杖中白藜芦醇的优化工艺研究[J]．食品工业科技，2008，29(7)：162-164．
[19]李梦青，聂媛，张洁，等．酶解法提取虎杖中白藜芦醇、白藜芦醇苷、大黄素[J]．精细化工，2008，25(5)：467-470．
[20]刘树兴，程丽英，陈志亮，等．白藜芦醇提取工艺的研究[J]．食品与发酵工业，2004，30(12)：145-147．
[21]郑湘娟，余淑娴，徐晓芳，等．紫外分光光度法测定虎杖中自藜芦醇的含量[J]．时珍国医国药，2008，19(8)：1881-1882．