高产γ-氨基丁酸霉菌菌株的筛选及诱变育种

食品科学 ›› 2012, Vol. 33 ›› Issue (21): 213-216.

高产γ-氨基丁酸霉菌菌株的筛选及诱变育种

边鑫，吴非*

东北农业大学食品学院

收稿日期:2011-10-21 修回日期:2012-10-16 出版日期:2012-11-15 发布日期:2012-11-09
通讯作者: 吴非 E-mail:wfneau@163.com
基金资助:
黑龙江省自然科学基金

Screening and Mutation Breeding of a High-Yield γ-Aminobuyric Acid(GABA) Mould Strain

Xin BIAN,

Received:2011-10-21 Revised:2012-10-16 Online:2012-11-15 Published:2012-11-09

摘要/Abstract

摘要： 以霉菌作为出发菌株，将其接入大豆-水(3:5，m/V)的发酵培养基中进行发酵，根据发酵过程中γ-氨基丁酸(GABA)产量，筛选出GABA的高产霉菌菌株，然后利用紫外线对高产菌株进行诱变处理，并通过抗性初筛获得长势较好的突变菌株，再分别利用含有质量浓度为1g/100mL L-谷氨酸的PDA综合培养基和大豆水溶液的发酵培养基进行两次筛选，得到稳定高产GABA突变菌株。结果表明，通过对产GABA霉菌菌株的筛选，得到米曲霉3.800为高产GABA霉菌，GABA产量达到0.674g/L。以米曲霉3.800作为原始菌株，对其进行紫外诱变处理，从而获得高产GABA突变菌株，结果表明，利用紫外线对米曲霉3.800进行处理的最佳照射时间为2min。在此照射时间下，通过突变菌株在含有质量浓度为1g/100mL L-谷氨酸的PDA综合培养基和大豆-水溶液的发酵培养基进行两次筛选，最终获得一株稳定的高产GABA突变菌株3.800-4，其在含有质量浓度为1g/100mL谷氨酸的PDA综合培养基中的GABA产量为4.491g/L，比原菌株的GABA产量提高了23.58%；同时其在大豆-水的发酵培养基中的GABA产量为0.874g/L，比原菌株的产量提高了29.67%。

关键词: γ-氨基丁酸, 霉菌, 筛选, 紫外诱变, 高产突变菌株

Abstract: In this study, different species of moulds were analyzed for effectiveness in fermenting cooked soybeans suspended in water (3:5, m/V) to produce γ-aminobuyric acid (GABA). As a result, Aspergillus oryzae 3.800 was found to have the highest GABA producing ability. The strain was mutagenized by UV irradiation for 2 min. For secondary screening, the resulting mutant strains were cultured initially in PDA medium containing 1 g/100 mL L-glutamic acid and then in soybean suspension. A stable high-yield mutant named as 3.800-4 was obtained. The GABA yield of the mutant was 4.491 g/L during culture in PDA medium containing 1 g/100 mL L-glutamic acid and 0.874 g/L during culture in soybean suspension, which was increased by 23.58% and 29.67%, respectively, compared with the original strain.

Key words: γ-aminobuyric acid, mould, screening, UV-induced mutagenesis, high-yield mutant strain

中图分类号:

理论与应用研究学术论文

边?鑫，吴?非*. 高产γ-氨基丁酸霉菌菌株的筛选及诱变育种[J]. 食品科学, 2012, 33(21): 213-216.

Xin BIAN. Screening and Mutation Breeding of a High-Yield γ-Aminobuyric Acid(GABA) Mould Strain[J]. FOOD SCIENCE, 2012, 33(21): 213-216.

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