食品科学 ›› 2013, Vol. 34 ›› Issue (6): 139-142.doi: 10.7506/spkx1002-6630-201306030

• 分析检测 • 上一篇    下一篇

多重PCR法检测转Bar、Bt基因双抗稻米

邱良焱1,肖有玉2,刘佳1,宋尚新1,肖红梅1,*,索娜1,王於建1,王雪强1   

  1. 1.南京农业大学食品科技学院,农业部农畜产品加工与质量控制重点开放实验室,江苏 南京 210095;2.南京市产品质量监督检验院,江苏 南京 210028
  • 收稿日期:2011-11-25 修回日期:2013-01-28 出版日期:2013-03-25 发布日期:2013-03-01
  • 通讯作者: 肖红梅 E-mail:xhm@njau.edu.cn
  • 基金资助:
    中央高校基本科研业务费项目

Multiplex PCR for detection of Transgenic Rice with Bar and Bt gene

QIU Liang-yan1,XIAO You-yu2,LIU Jia1,SONG Shang-xin1,XIAO Hong-mei1,*,SUO Na1,WANG Yu-jian1,WANG Xue-qiang1   

  1. 1. Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture, College of Food Science and Technology, Nanjing AgriculturalUniversity, Nanjing 210095, China;2. Nanjing Institute of Supervision & Testing on Product Quality, Nanjing 210028, China
  • Received:2011-11-25 Revised:2013-01-28 Online:2013-03-25 Published:2013-03-01

摘要: 旨在建立一种快速、有效的检测转基因双抗稻米的方法,以转Bar、Bt基因双抗稻米为原料,针对其水稻内源基因SPS和外源基因(包括Bar基因、Bt基因、CaMV35S启动子、Ubiquitin启动子和NOS终止子)设计多重PCR检测体系。结果表明,应用该多重PCR检测体系可快速检测出原料中的全部6条目标基因,检测灵敏度可达0.9%。

关键词: 转基因水稻, 检测, 多重PCR

Abstract: Based on the DNA sequences of rice endogenous SPS gene and exogenous Bar, Bt, CaMV35S promoter, Ubiquitin promoter and NOS terminator genes, specific primers were selected to establish a multiplex polymerase chain reaction (PCR) detection system for transgenic rice with Bar and Bt genes. This multiplex PCR system could allow accurate and rapid detection of six target genes, and the sensitivity was 0.9%.

Key words: transgenic rice, detection, multiplex PCR

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