食品科学 ›› 2013, Vol. 34 ›› Issue (6): 215-218.doi: 10.7506/spkx1002-6630-201306048

• 分析检测 • 上一篇    下一篇

超高效液相色谱飞行时间质谱测定食品中36种合成色素

钱疆1,杨方1,*,陈弛2,陈健1,张玉燕1,陈章捷1,薛芝敏1,余孔捷1   

  1. 1.福建出入境检验检疫局,福建 福州 350001;2.福建农林大学食品科学学院,福建 福州 350002
  • 收稿日期:2011-11-27 修回日期:2013-01-24 出版日期:2013-03-25 发布日期:2013-03-01
  • 通讯作者: 杨方 E-mail:yf@fjciq.gov.cn
  • 基金资助:
    福建省自然科学基金重点项目;福建检验检疫局科技项目

Determination of 36 Artificial Pigments in Foods by Ultra High Performance Liquid Chromatography and Time-of-Fight Mass Spectrometry

QIAN Jiang1,YANG Fang1,*,CHEN Chi2,CHEN Jian1,ZHANG Yu-yan1,CHEN Zhang-jie1,XUE Zhi-min1,YU Kong-jie1   

  1. 1. Fujian Enrty-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China;2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2011-11-27 Revised:2013-01-24 Online:2013-03-25 Published:2013-03-01

摘要: 建立食品中36种合成色素同时测定方法。样品经热水提取,采用聚酰胺粉净化,超高效液相色谱用甲醇-5mmol/L乙酸铵溶液梯度淋洗,1.8μm C18短柱分离,在电喷雾离子源负离子模式下采用飞行时间质谱检测。36种合成色素线性范围在0.01~1μg/mL,检测低限在0.0072~0.94mg/kg之间,加标回收率在80%~106%范围,标准偏差在1.8%~9.7%之间。

关键词: 飞行时间质谱, 超高效液相色谱, 合成色素, 食品, 高通量

Abstract: An ultra high performance liquid chromatography and time-of-flight mass spectrometry (UPLC-TOF-MS) method for the determination of 36 artificial pigments in foods was established. Samples were extracted with hot water and purified with polyamide powder, and the extract was separated on C18 column (1.8 μm) using methanol-5 mmol/L ammonium acetate as the mobile phrase through gradient elution and analyzed by UPLC-TOF-MS coupled with electrospray ionization (ESI) resource in a negative ion mode. An excellent linear relationship for pigments was observed in the concentration range of 0.01–1 μg/mL and the detection limit was 0.0072–0.94 mg/kg. The recovery rate of spiked samples was in the range of 80%–106% with relative standard deviation of 1.8%–9.7%.

Key words: time-of-flight mass spectrometry (TOF-MS), ultra pressure liquid chromatography (UPLC), artificial pigment, food, high throughout

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