食品科学 ›› 2012, Vol. 33 ›› Issue (18): 10-13.

• 工艺技术 • 上一篇    下一篇

碱溶性山核桃蛋白的提取及SDS-PAGE分析

于阳阳,包怡红*   

  1. 东北林业大学林学院
  • 收稿日期:2011-07-11 修回日期:2012-07-25 出版日期:2012-09-25 发布日期:2012-09-07
  • 通讯作者: 于阳阳 E-mail:yuyangyang.1986@163.com
  • 基金资助:

    中央高校基金

Extraction and SDS-PAGE Analysis of Alkali-Soluble Proteins from Juglans mandshurica Maxim Kernels

Yang-Yang YU,BAO Yi-hong   

  • Received:2011-07-11 Revised:2012-07-25 Online:2012-09-25 Published:2012-09-07
  • Contact: Yang-Yang YU E-mail:yuyangyang.1986@163.com

摘要: 以经超临界CO2去除油脂后的东北山核桃仁渣为原料,利用碱提法对山核桃渣进行蛋白质提取,考察NaOH溶液浓度、料液比、提取温度、提取时间4个因素对蛋白提取率的影响,并在单因素试验的基础上设计正交试验,确定碱溶性山核桃仁蛋白最佳提取条件和其等电点。同时对碱溶性山核桃蛋白进行SDS-PAGE凝胶电泳分析。结果表明:当提取液NaOH浓度0.02mol/L、料液比1:30(g/mL)、提取温度50℃、提取时间1.5h时,碱溶性山核桃蛋白提取率最大可达88.05%,其等电点为4.5。SDS-PAGE分析结果表明,碱溶性山核桃蛋白分子质量主要分布在100、60、36kD和20kD 四个区域。

关键词: 山核桃, 碱溶性蛋白, 提取, SDS-PAGE, 分子质量

Abstract: The extraction of alkali-soluble proteins from Juglans mandshurica Maxim kernel waste left over after oil extraction with supercritical carbon dioxide was optimized using one-factor-at-a-time and orthogonal array design methods. The effects of NaOH concentration, material-to-solvent ratio, temperature and extraction time on the extraction yield of protein. It was found that the highest extraction yield of protein, 88.05%, was obtained after 1.5 h of extraction with 0.02 mol/L NaOH solution at a material-to-solvent ratio of 1:30 and 50 ℃. The isoelectric point of the extract obtained under these conditions was measured to be 4.5. SDS-PAGE analysis showed that the molecular weight distribution of alkali-soluble proteins from Juglans mandshurica Maxim kernels was mainly 100, 60, 36 kD and 20 kD.

Key words: Juglans mandshurica Maxim, alkali-soluble protein, extraction, SDS-PAGE, molecular weight

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