软枣猕猴桃多糖的分离与纯化研究及其分子量的测定

食品科学 ›› 2012, Vol. 33 ›› Issue (15): 66-70.

软枣猕猴桃多糖的分离与纯化研究及其分子量的测定

潘松，刘长江*，梁爽

沈阳农业大学食品学院

收稿日期:2011-07-11 修回日期:2012-06-20 出版日期:2012-08-15 发布日期:2012-09-07
通讯作者: 刘长江 E-mail:liucj597@sohu.com
基金资助:
农业部公益性行业（农业科研专项）

Isolation, Purification and Molecular Weight Determination of Polysaccharides from

Received:2011-07-11 Revised:2012-06-20 Online:2012-08-15 Published:2012-09-07

摘要/Abstract

摘要： 对通过水提醇沉法提取、Sevag法脱蛋白得到的软枣猕猴桃多糖进行分离纯化，利用DEAE-纤维素 52离子交换层析对软枣猕猴桃多糖进行初步分离，得到4种软枣猕猴桃多糖组分；利用葡聚糖凝胶对软枣猕猴桃多糖组分进一步分离的最佳条件为葡聚糖凝胶型号为Sephadex G-200、层析柱规格为D1.1cm×100cm、多糖质量浓度为10g/L。以此条件分离，得到含量较高的软枣猕猴桃多糖-Ⅱ纯品。通过Sephadex G-200凝胶柱层析鉴定软枣猕猴桃多糖-Ⅱ是均一的纯多糖，通过紫外光谱鉴定软枣猕猴桃多糖-Ⅱ不含核酸及蛋白。同时测得软枣猕猴桃多糖-Ⅱ的分子质量为83733D。

关键词: 软枣猕猴桃, 多糖, 分离纯化, 柱层析, 紫外光谱, 分子质量测定

Abstract: Polysaccharides from Actinidia arguta Sieb. et Zucc. were extracted by hot-water extraction, ethanol precipitation, protein removal by the Sevag method and then purified by DEAE-cellulose-52 ion exchange column chromatography. Four polysaccharide fractions were obtained. The best purification of Fraction Ⅱ was achieved by chromatographic separation on a Sephadex G-200 column (D1.1 cm × 100 cm) at a sample concentration of 10 g/L. As a result, a homogenous pure polysaccharide was obtained. The results of UV spectroscopic analysis revealed that the purified polysaccharide contained neither proteins nor nucleic acids. Moreover, the molecular weight of the purified polysaccharide was measured to be 83733 D.

Key words: Actinidia arguta Sieb. et Zucc., polysaccharide, isolation and purification, column chromatography, ultraviolet spectroscopy, molecular weight determination

中图分类号:

TS255.36

潘松，刘长江*，梁爽. 软枣猕猴桃多糖的分离与纯化研究及其分子量的测定[J]. 食品科学, 2012, 33(15): 66-70.

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