食品科学 ›› 2012, Vol. 33 ›› Issue (20): 1-6.

• 工艺技术 •    下一篇

响应面分析法优化蜂胶黄酮脂质体制备工艺

袁 菊,张亚宁,范云鹏,胡元亮,王远垒,赵晓娟,王德云*   

  1. 南京农业大学中兽医学研究室
  • 收稿日期:2011-07-21 修回日期:2012-08-10 出版日期:2012-10-25 发布日期:2012-11-09
  • 通讯作者: 袁菊 E-mail:wodejuer@163.com
  • 基金资助:

    国家自然科学基金项目

Optimization of Preparation Conditions for Propolis Derived Flavonoid Liposomes by Response Surface Methodology

  • Received:2011-07-21 Revised:2012-08-10 Online:2012-10-25 Published:2012-11-09

摘要: 目的:优选蜂胶黄酮脂质体制备的的最佳条件。方法:采用乙醇注入法制备蜂胶黄酮脂质体,在单因素试验基础上,以脂药比、膜材比、注入速度为影响因素,包封率为响应值,利用Box-Behnken试验设计原理,采用3因素3水平的响应面分析法,对蜂胶黄酮脂质体制备工艺进行优化。结果:蜂胶黄酮脂质体的最佳制备条件为脂药比9.6:1、膜材比8.5:1、注入速度0.8mL/min。结论:采用最佳条件制备的蜂胶黄酮脂质体包封率较高,验证实验测得蜂胶黄酮脂质体的包封率为91.67%,与模型预测值相对误差为0.086%,重现性好。

关键词: 响应面分析, 蜂胶黄酮脂质体, 包封率, 乙醇注入法, 脂药比

Abstract: Objective: To optimize conditions for the preparation of propolis derived flavonoid (PF) liposome. Methods: PF liposomes were prepared by ethanol injection method. One-factor-at-a-time design was used to investigate the effects of five process parameters on encapsulation efficiency. Three main process parameters that influence encapsulation efficiency including lecithin/PF mass ratio, lecithin/cholesterol mass ratio and injection speed were optimized by response surface analysis based on a three-variable, three-level Box-Behnken experimental design. Results: The optimal conditions for preparing PF liposomes were determined as 9.6:1 of lecithin/PF mass ratio, 8.5:1 of lecithin/cholesterol mass ratio and 0.8 mL/min of injection speed. Conclusion: Under these conditions, the actual encapsulation efficiency of PF liposomes was 91.67%, which showed a relative error of 0.086% when compared with the predictive value.

Key words: response surface methodology, propolis derived flavonoid liposome, encapsulation efficiency, ethanol injection method, lecithin/PF mass ratio

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