蚕蛹油对三种不同类型肝损伤小鼠的保护作用

摘要/Abstract

摘要： 目的：研究蚕蛹油对3种不同类型的肝损伤小鼠的保护作用。方法：雄性ICR小鼠150只，按体质量随机分为3大组：实验a组(酒精性肝损伤组)、实验b组(免疫性肝损伤组)、实验c组(D-半乳糖胺肝损伤组)。每大组再随机分为5个小组：空白对照组、月见草油阳性对照组、肝损伤模型组及蚕蛹油低、高剂量组。灌胃7d后，除空白对照组外，各实验组分别进行如下处理：实验a组给予50%乙醇12mL/kg，建立小鼠酒精性肝损伤模型；实验b组采用尾静脉注射卡介苗(BCG)＋脂多糖(LPS)诱导小鼠免疫性肝损伤；实验c组以10% D-半乳糖胺600mg/kg给各组小鼠腹腔注射，诱导小鼠D-半乳糖胺肝损伤模型。24h后测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)的活性和肝组织中丙二醛(MDA)、还原型谷胱甘肽(GSH)的含量；观察肝脏、脾脏指数的变化。结果：蚕蛹油组肝脏指数、脾脏指数、ALT和AST的活性及肝组织中MDA、还原型GSH水平与模型组各水平相比均具有显著性差异(P＜0.05)，且肝脏、脾脏指数水平与ALT、AST活性及MDA、GSH含量相关性显著(P＜0.05)。结论：蚕蛹油对酒精所致小鼠急性化学性肝损伤、卡介苗和脂多糖诱导的免疫性肝损伤、D-半乳糖胺所造成的小鼠肝损伤均具有明显的保护作用，且对酒精性肝损伤及BCG和LPS诱导的免疫性肝损伤保护效果优于月见草油，其机制可能与其降酶、降低脂质过氧化水平、阻止肝细胞坏死作用有关。

关键词: 蚕蛹油, 多不饱和脂肪酸, 肝损伤, 脂质过氧化

Abstract: Objective: To investigate the protective effect of chrysalis oil against three different types of liver injury in mice. Methods: A total of 150 male ICR mice were equally randomized into 3 groups by weight: group a (acute alcoholic hepatic injury group), group b (immunological liver injury group), and group c (D-galactosamine-induced liver injury group). Then the mice in each group were further randomly assigned into 5 subgroups: normal control, evening promise oil, liver injury control, low and high-dose chrysalis oil subgroups. After administration for 7 days, the mice in each group (except mice in the normal control subgroup) were treated as follows: the mice in group a were given 50% ethanol (12 mL/kg) to establish a mouse model of alcoholic liver injury; the mice in group b were intravenously injected with Bacille de Calmette Guerin (BCG) + lipidpolysaccharide (LPS) to induce a mouse model of immune liver injury; the mice in group c were intraperitoneally injected with D-GAL (600 mg/kg) to induce a mouse model of acute liver injury. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and liver malondialdehyde (MDA) and glutathione (GSH) content were measured 24 hours later. Results: The liver and spleen indexes, serum ALT and AST activities, and MDA and GSH content in the liver of mice from the chrysalis oil groups were significantly different to those of the liver injury control group (P ＜ 0.05). The liver and spleen indexes were significantly correlated with ALT and AST activities in the blood and MDA and GSH levels in the liver (P ＜ 0.05). Conclusion: Chrysalis oil has a significant protective effect against ethanol induced acute liver injury, BCG and LPS induced liver injury and D-GAL induced liver injury in mice. Its protective effect against alcoholic liver injury and BCG+LPS induced liver injury is greater than that of evening primrose oil. The mechanism may be related to its property to reduce lipid peroxidation in the body and its ability to prevent liver cells from necrosis.

Key words: chrysalis oil, polyunsaturated fatty acids, liver injury, lipid peroxidation

中图分类号:

陈伟平1，谢园沁2，胡金鹿2. 蚕蛹油对三种不同类型肝损伤小鼠的保护作用[J]. 食品科学, 2012, 33(15): 291-296.

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