食品科学 ›› 2012, Vol. 33 ›› Issue (20): 343-346.

• 技术应用 • 上一篇    下一篇

原料乳蛋白质含量及酸度多功能快速检验试剂盒的研制

张桂春,辛晓林,张玉香,刘进杰   

  1. 鲁东大学生命科学学院
  • 收稿日期:2011-07-31 修回日期:2012-08-27 出版日期:2012-10-25 发布日期:2012-11-09
  • 通讯作者: 张桂春 E-mail:gczhshl@163.com

Development of Rapid Multifunctional Assay Kit for the Determination of Protein Content and Acidity in Raw Milk

  • Received:2011-07-31 Revised:2012-08-27 Online:2012-10-25 Published:2012-11-09

摘要: 将原料乳蛋白质含量测定技术和酸度测定技术试剂盒化。采用甲醛滴定法测定原料乳蛋白质含量、酸碱滴定法测定原料乳酸度,确定试剂盒的组分,并对试剂盒的符合率、重复性、稳定性等进行研究。该试剂盒由4种试剂组成,与凯氏定氮法和酸碱滴定法的符合率为100%,重复性良好,密封干燥处保存540d各项性能仍然很好。该试剂盒既可同时检测各种原料乳蛋白质含量和酸度,也可检测各种消毒乳和酸牛乳的蛋白质含量。

关键词: 原料乳, 蛋白质含量, 酸度, 快检, 试剂盒

Abstract: In this study, an integrative array kit for rapid simultaneous determination of protein content and acidity in raw milk was developed based on formaldehyde titration and acid-base titration. The kit was composed of 4 regents. The results obtained with it, highly stable, were completely consistent with those obtained by the Kjeldahl method and acid-base titration. After 540 d of sealed dry storage, its performance remained good. This kit may be also available for the determination of protein content in sterilized milk and yogurt.

Key words: raw milk, protein content, acidity, rapid determination, kit

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