食品科学 ›› 2013, Vol. 34 ›› Issue (2): 143-147.

• 分析检测 • 上一篇    下一篇

响应面法优化超声波辅助提取湖北海棠叶中总黄酮工艺

乔孟1,丁之恩2   

  1. 1. 安徽农业大学
    2. 安徽农业大学茶与食品科技学院
  • 收稿日期:2011-11-14 修回日期:2012-12-09 出版日期:2013-01-25 发布日期:2013-01-15
  • 通讯作者: 丁之恩 E-mail:dingze08@hotmail.com

Optimization of Ultrasonic-assisted Extraction of Total Falconoids from Malus hupehensis by Response Surface Method

  • Received:2011-11-14 Revised:2012-12-09 Online:2013-01-25 Published:2013-01-15

摘要:

采用超声波辅助提取湖北海棠叶中总黄酮,并利用响应面法对提取工艺参数进行优化(研究)。在料液比,乙醇体积分数,超声时间,超声功率等单因素实验基础上,进行四因素三水平的Box-Behnken中心组合设计,利用响应面法分析4个因素对湖北海棠叶中总黄酮得率的影响。超声波法提取湖北海棠叶中总黄酮的最佳工艺条件为:液料比50:1(mL/g),乙醇体积分数为67.35 %,超声时间为3.2 h,超声功率为162.4 W,总黄酮得率预测值为12.78 %,验证值为12.76 %,与理论值的相对误差为0.02 %,表明该优化方法合理可行。

Abstract:

Response surface analysis methodology (RSM) was applied to optimize the ultrasonic-assisted extraction conditions of total flavonoids from Malus hupehensis. The concentrations of ethanol in solvent, extraction time, ultrasonic power and ratio of liquid to solid were chosen as casual factors. Base on the single-factor experiments four-factor-three-levels box behnken experiments design were applied. RSM was employed to study on the effect of these factors on the yield of total flavonoids from Malus hupehensis. The results show that the optimum ultrasonic-assisted conditions were ratio of liquid to solid 50:1(mL/g), 67.35 % ethanol, extracting time 3.2 h, ultrasonic power 162.4 W, Validation experiments were carried out at these optimum conditions. The predicted value of the maximal yield of flavonoids is 12.78 %, while the effective value is 12.76 %, the relative error between actual values and predicted values is 0.02 %. These results demonstrated that the method is feasible.

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