食品科学 ›› 2013, Vol. 34 ›› Issue (4): 109-114.

• 工艺技术 • 上一篇    下一篇

响应面法优化纤维素酶提取山核桃蒲多酚类物质

董旭1,2   

  1. 1. 安徽农业大学茶与食品科技学院
    2. 安徽省农业科学院植物保护与农产品质量安全研究所
  • 收稿日期:2012-02-08 修回日期:2013-01-14 出版日期:2013-02-25 发布日期:2013-01-29
  • 通讯作者: 董旭 E-mail:dongxu929@163.com

RSM of Optimized Cellulase Extraction of polyphenol from Carga cathayensis Sarg peels

  • Received:2012-02-08 Revised:2013-01-14 Online:2013-02-25 Published:2013-01-29

摘要:

利用纤维素酶法提取山核桃蒲多酚,在单因素的基础上,通过SAS数据统计分析软件,运用四因素三水平的响应面设计方法,建立纤维素酶法提取山核桃蒲多酚的二次多项数学模型,以提取率为响应值作响应面和等高线,最优浸提条件为:pH为6,浸提温度为70℃、加酶量为4U/g底物、浸提时间70min,此时的提取率预测值为63.43%,实际重复得出该条件下提取率为61.72%,相对偏差为2.77%。

Abstract:

Response surface methodology was employed to optimize the extraction condition of polyphenols from carga cathayensis sarg peels treated with cellulase. On the basis of one-factor-at-a-time experiments, a four-factor, three-level quadratic general rotary unitization design was employed to establish a quadratic polynomial mathematical model that describes extracting rate as a function of four process conditions, and the established model was analyzed by drawing response surface and contour plots. The optimal extraction conditions were determined as follows: 4U/g substrate at pH 6 and 70℃ for 70min. Under these conditions, the extracting rate of carga cathayensis sarg peels was predicted to 63.43% and observed to 61.72% with a relative error of 2.77%.

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