食品科学 ›› 2013, Vol. 34 ›› Issue (3): 257-260.

• 营养卫生 • 上一篇    下一篇

海带多糖硫酸酯对bFGF诱导的血管平滑肌细胞增殖的影响

刘敏,彭臻菲,方哲翔,张其清,许小平   

  1. 福州大学生物和医药技术研究院
  • 收稿日期:2011-11-21 修回日期:2013-01-05 出版日期:2013-02-15 发布日期:2017-12-29
  • 通讯作者: 张其清 E-mail:zhangqiq@126.com
  • 基金资助:
    福建省科技重大专项专题

Effects Sulfated of Polysaccharides Extracted from Laminaria Japonica on Proliferation of Vascular Smooth Muscle Cells Induced by BFGF

  • Received:2011-11-21 Revised:2013-01-05 Online:2013-02-15 Published:2017-12-29

摘要: 目的:研究海带多糖硫酸酯WPS、WPS-1、WPS-2和WPS-3对碱性成纤维细胞生长因子(bFGF)诱导的大鼠主动脉血管平滑肌细胞(VSMC)增殖的影响。方法:采用组织贴块法培养VSMC并用免疫组织化学染色法对VSMC进行鉴定;采用bFGF诱导VSMC增殖;以MTT法检测海带多糖硫酸酯对bFGF诱导的VSMC增殖的影响;黄嘌呤/黄嘌呤氧化酶产超氧阴离子自由基体系测定海带多糖硫酸酯对超氧自由基(O2-?)的清除力。结果:在0.1-1 mg/mL浓度范围内,WPS、WPS-1和WPS-3对bFGF诱导的VSMC增殖具有抑制作用,其中WPS的增殖抑制性表现显著。 WPS-2则对VSMC增殖基本无影响。在O2-?清除力上,WPS-2对O2-?清除率仅为34%,而其他组分自由基清除率均超过70%。结论:WPS、WPS-1和WPS-3对bFGF诱导的VSMC增殖均有显著抑制作用,且抑制作用可能与它们的自由基清除能力相关。

Abstract: Objective: To investigate the inhibitory effects of sulfated polysaccharides from Laminaria japonica on rat vascular smooth muscle cells (VSMCs) induced by basic fibroblast growth factor (bFGF). Methods: Rat VSMCs were obtained from the aorta of adult Sprague Dawley rats by attached explant method and identified by immunohistochemistry assay. VSMCs proliferation was induced by basic fibroblast growth factor (bFGF). The inhibitory effects of crude sulfated polysaccharide and its fractions on VSMCs proliferation were evaluated by MTT assay. Superoxide anion-scavenging activities of sulfated polysaccharides were investigated by xanthine /xanthine oxidase method. Results: At the concentration of 0.1-1 mg/mL, all sulfated polysaccharides dose-dependently inhibited bFGF-induced VSMCs proliferation except WPS-2. Similarly, all sulfated polysaccharides showed good superoxide radical scavenging capabilities except WPS-2. Conclusion: All sulfated polysaccharides can dramatically inhibit the proliferation of VSMCs induced by bFGF except WPS-2. The functional mechanism may be related to their antioxidant activities.

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