液相色谱-串联质谱测定食用植物油和调味油中27种添加剂（物）

食品科学 ›› 2013, Vol. 34 ›› Issue (4): 129-134.

液相色谱-串联质谱测定食用植物油和调味油中27种添加剂（物）

曹娅

中国检验检疫科学研究

收稿日期:2011-11-25 修回日期:2013-01-14 出版日期:2013-02-25 发布日期:2013-01-29
通讯作者: 曹娅 E-mail:xf880419@126.com
基金资助:
国家科技支撑计划;国家质检总局科技计划;北京市科技计划项目

Simultaneous Determination of 27 kinds of food additives and illegal additives in vegetable oil and seasoning oil by High-Performance Liquid Chromatography- Tandem Mass Spectrometry

Received:2011-11-25 Revised:2013-01-14 Online:2013-02-25 Published:2013-01-29

摘要/Abstract

摘要：

建立了食用植物油和调味油中18种食品添加剂和9种非法添加物的液相色谱-串联质谱（HPLC-MS/MS）分析方法。样品经正己烷稀释后，采用乙腈振荡提取，乙腈层定容后直接测定18种添加剂；正己烷层浓缩后采用凝胶渗透色谱（GPC）除去油脂，洗脱液浓缩后进行9种非法添加物的分析。在电喷雾电离（ESI）模式下采用多反应监测模式（MRM）进行测定，27种添加剂（物）在各自浓度范围内线性良好，线性相关系数>0.983。空白样品添加的回收率在78.2-108.3%之间；精密度为0.9-17.7%。将该方法应用于市售样品的检测，发现五个样品中含有抗氧化剂TBHQ，但是均低于我国限量要求。

Abstract:

A high performance liquid chromatography- tandem mass spectrometry（HPLC- MS/MS）method for determination of 18 kinds of food additives and 9 kinds of illegal additives in vegetable oil and seasoning oil has been developed. Samples were diluted by hexane, extracted by acetonitrile and the acetonitrile layer was made up to constant volume before tested the 18 kinds of food additives. Hexane layer was concentrated before it cleansing through GPC, and the eluent was concentrated, made up to constant volume, at last the treated samples used HPLC-MS/MS assay. In the electrospray ion mode and MRM mode，27 kinds of compounds in the range show a good linear relationship, linear coefficients were greater than 0.983. The average recoveries ranged from 78.2% to 108.3%, the relative standard deviation is within0.9%-17.7%.This method can be used for testing the food additives and illegal additives in vegetable oil and seasoning oil. Applied this method in market samples detection，we found TBHQ in five kinds of samples，but，all of them was below the maximum limit in China.

中图分类号:

TS202.3

曹娅. 液相色谱-串联质谱测定食用植物油和调味油中27种添加剂（物）[J]. 食品科学, 2013, 34(4): 129-134.

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