食品科学 ›› 2013, Vol. 34 ›› Issue (3): 109-114.

• 基础研究 • 上一篇    下一篇

4种葡萄抗氧化活性比较

敬思群1,马泽鑫1,2,欧阳位麒1,相恒绪1,任志艳1   

  1. 1. 新疆大学生命科学与技术学院
    2. 巴州产品质量检验所
  • 收稿日期:2012-02-09 修回日期:2013-01-11 出版日期:2013-02-15 发布日期:2017-12-29
  • 通讯作者: 敬思群 E-mail:jingsiqun@163.com

Study on antioxidant activity of four kind of grape

  • Received:2012-02-09 Revised:2013-01-11 Online:2013-02-15 Published:2017-12-29
  • Contact: Siqun Jing E-mail:jingsiqun@163.com

摘要: 本文通过测定总抗氧化能力,清除DPPH?能力,清除羟自由基(?OH)能力及清除超氧阴离子自由基(O2-?)的能力,评价四种葡萄汁三种提取物的体外抗氧化能力。通过动物实验评价玫瑰香葡萄汁水提物体内抗氧化活性,同时定性、定量分析了玫瑰香葡萄汁水提物的活性成分。结果表明:四种葡萄汁的同一种提取物的体外抗氧化能力显著差异。综合分析玫瑰香葡萄汁水提物体外抗氧化能力最强。剂量800mg/(kg bw?d)玫瑰香葡萄汁水提物能显著提高小鼠血清及肝脏组织中超氧化物歧化酶(SOD)酶活力,降低肝脏组织中丙二醛(MDA)含量。玫瑰香葡萄汁水提物中含有多糖,有机酸,黄酮类,酚类等抗氧化活性物质,其中总酚含量4.56mg/10ml,总黄酮4.37 mg/ml,

Abstract: In this paper,by measuring the total antioxidant capacity, scavenging DPPH ? ability,scavenging ? OH and O2-? ability to evaluate antioxidant capacity of the three extract of four grape juice in vitro. By animal experiments to evaluate antioxidant capacity of the AE extracting from the Muscat grape juice in vivo ,and by chemical qualitative and quantitative analysis the active ingredient of the AE from the Muscat grape juice. The results showed that: there were significantly difference among the same extracts of different kind of grape juice on antioxidant activity aspect in vitro. The vitro antioxidant capacity of the AE from the Muscat grape juice was strongest. The AE [800mg / (kg bw ? d) ] from the Muscat grape juice could increase serum and liver tissue SOD activity ,and reduce the MDA content in liver tissue. The AE from the Muscat grape juice contains polysaccharides, organic acids, flavonoids, polyphenols and other antioxidant substances,which have total phenolics 0.456mg/ml ,total flavonoids4.37 mg/ml.

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