食品科学 ›› 2013, Vol. 34 ›› Issue (3): 184-187.

• 生物工程 • 上一篇    下一篇

抗玉米赤霉烯酮单抗独特型抗体制备与鉴定

余涛1,邓宁1,宋其芳1,贾彦琼2,王宏2   

  1. 1. 暨南大学生命科学技术学院抗体工程研究中心
    2. 暨南大学抗体工程中心
  • 收稿日期:2011-11-17 修回日期:2012-12-29 出版日期:2013-02-15 发布日期:2017-12-29
  • 通讯作者: 余涛 E-mail:yutaopengwa123@126.com
  • 基金资助:
    “十一五”国家科技支撑计划项目;天河区科技计划项目;暨南大学创新基金资助

Preparation and Identification of Anti-idiotypic Antibody against Anti-Zearalenone Monoclonal Antibody

  • Received:2011-11-17 Revised:2012-12-29 Online:2013-02-15 Published:2017-12-29

摘要: 目的:制备抗玉米赤霉烯酮(ZEN)单抗独特型抗体,为建立ZEN无毒检测方法奠定基础。方法:在研制了抗ZEN单抗的基础上,将单抗与载体蛋白KLH偶联作为免疫原,免疫BALB/c小鼠,同时用胃蛋白酶将单抗进行酶切,获得抗体Fab片段,作为检测抗独特型抗体的包被原,利用间接竞争ELISA研究独特型抗体特性。结果 :所研制的抗独特型抗体与ZEN毒素之间存在内影像关系,相关系数r值达到0.99。结论:成功制备了抗ZEN单抗独特型抗体,可以替代ZEN毒素标准品应用于ELISA检测。

Abstract: Abstract:Purpose:To lay the foundation for develop the toxin-free detection method for zearalenone(ZEN), an anti-idiotypic antibody(AId) against anti-zearalenone toxin monoclonal antibody(McAb) was prepared.Methods:The polyclonal anti-idiotype antibody against anti-ZEN McAb was prepared by immunizing BALB/c mouse with the conjugate McAb - KLH ,and the AId was detected by ELISA with Fab fragment as coating antigen.Results:The obtained AId against anti-ZEN McAb was identified as the“inner image”with ZEN toxin,and the correlation coefficient reached to 0.99.Conclusion:The polyclonal AId against anti-ZEN McAb was prepared successfully and could be used to replace ZEN toxin standard in ELISA. 

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