关键词: 高效液相色谱法, 食品, 食品添加剂

Abstract: A high performance liquid chromatography method was developed for simultaneous determination of 18 food additives in foods. Low fat or fat free food samples were extracted and purified with n-hexane saturated acetonitrile containing ascorbyl palmitate-6 mol/L HCl solution-saturated sodium chloride solution (containing medium fat sample usingnhexane to remove fat). Oil samples were extracted with n-hexane saturated acetonitrile (containing ascorbyl palmitate)- acetonitrile saturated n-hexane. Analysis was performed using HPLC system equipped with an Ecosil C18 column (250 mm × 4.6 mm, 5 μm) and a UV detector at 280 nm with a mobile phase of acetonitrile-0.6% acetic acid solution, at a flow rate of 1.0 mL/min with gradient elution. Good linear was observed in the range of 1.0–25 mg/L with correlation coefficient of 0.99. The average recovery rate in foods was in the range of 88.9%–99.9% at three spiked levels of 10, 25 mg/kg and 50 mg/kg (n = 6), and the relative standard deviation was in the range of 2.43%–11.7%. The limit of quantification was 10 mg/kg. This method is simple, accurate and suitable for the determination of 18 food additives in foods.

Key words: high performance liquid chromatography (HPLC), foods, food additives