食品科学 ›› 2013, Vol. 34 ›› Issue (5): 137-141.doi: 10.7506/spkx1002-6630-201305029

• 生物工程 • 上一篇    下一篇

β-胡萝卜素降解葡萄球菌化学合成培养基营养素的研究

麻俊侠1,樊明涛1,*,王树林2,朱明明1   

  1. 1.西北农林科技大学食品科学与工程学院,陕西 杨凌 712100;2.青海大学农牧学院,青海 西宁 810016
  • 收稿日期:2012-01-03 修回日期:2013-01-14 出版日期:2013-03-15 发布日期:2013-04-16
  • 通讯作者: 樊明涛 E-mail:fanmt@nwsuaf.edu.cn
  • 基金资助:
    细菌中类胡萝卜素降解关键酶基因克隆与酶降解机理研究;沙棘源微生物降解类胡萝卜素产香的酶学特性及酶编码基因的异源表达研究

Screening of Nutrients in Chemically Defined Medium for β-Carotene-Degrading Staphylococcus sp.

MA Jun-xia1,FAN Ming-tao1,*,WANG Shu-lin2,ZHU Ming-ming1   

  1. 1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; 2. Agriculture and Animal Husbandry College, Qinghai University, Xining 810016, China
  • Received:2012-01-03 Revised:2013-01-14 Online:2013-03-15 Published:2013-04-16
  • Contact: FAN Ming-tao E-mail:fanmt@nwsuaf.edu.cn

摘要: 目的:探索适于β-胡萝卜素降解葡萄球菌生长的最佳化学合成培养基配方。方法:先确定培养基成分,再利用单一成分缺失方法确定β-胡萝卜素降解葡萄球菌的必需营养素。结果:K2HPO4、NaNO3、Pro、Arg、Val、Gly、Try等物质是此菌体生长必需的营养素。结论:经筛选合成的完全化学合成培养基可以用于此β-胡萝卜素降解葡萄球菌的培养,培养物具有降解类胡萝卜素能力。

关键词: 必需营养素, 单一缺失法, 化学合成培养基, β-胡萝卜素降解

Abstract: Objective: To explore the optimum synthetic medium suitable for β-carotene-degrading Staphylococcus sp.. Methods: The medium composition was determined and essential nutrients for β-carotene-degrading Staphylococcus sp. were chosen by single component deficiency method. Results: K2HPO4, NaNO3, proline, arginine, valine, glycine and tryptophan were essential for the growth of β-carotene-degrading Staphylococcus sp.. Conclusion: The optimized synthetic medium is suitable for the cultivation of β-carotene-degrading Staphylococcus sp. to produce β-carotene-degrading materials.

Key words: essential nutrients, single component deficiency, synthetic medium, β-carotene degradation

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