食品科学 ›› 2013, Vol. 34 ›› Issue (8): 153-156.doi: 10.7506/spkx1002-6630-201308031

• 分析检测 • 上一篇    下一篇

高效液相色谱法定量分析茶籽粕中的茶皂素

张海龙,张维农,胡志雄,齐玉堂,龚 任   

  1. 1.武汉工业学院食品科学与工程学院,湖北 武汉 430023;2.武汉工业学院分析测试中心,湖北 武汉 430023;3.国家粮食局武汉科学研究设计院,湖北 武汉 430070
  • 收稿日期:2012-01-03 修回日期:2013-03-14 出版日期:2013-04-25 发布日期:2013-05-07
  • 通讯作者: 张海龙 E-mail:yinghexingshi@163.com
  • 基金资助:
    国家863项目;武汉工业学院食品营养与安全研究院重大项目培育专项目

Quantitative Determination of Tea Saponin in Tea Seed Meal by High Performance Liquid Chromatography

ZHANG Hai-long,ZHANG Wei-nong,HU Zhi-xiong,QI Yu-tang,GONG Ren   

  1. 1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;2. Analysis and Test Center, Wuhan Polytechnic University, Wuhan 430023, China;3. Wuhan Science Research and Design Institute, State Administration of Grain, Wuhan 430070, China
  • Received:2012-01-03 Revised:2013-03-14 Online:2013-04-25 Published:2013-05-07

摘要: 以脱脂冷榨茶籽饼粕为原料,采用反相ODS填料纯化制备茶皂素标准品,并采用紫外吸收光谱、红外光谱及质谱对该标准品进行鉴定;采用自制标准品为标样,建立一种高效液相色谱法定量分析茶籽饼粕中茶皂素含量的方法。高效液相色谱法条件为反相ODS色谱柱、检测波长215nm、柱温室温、流速1.0mL/min、流动相以乙腈-水体系进行梯度洗脱(0~5min内乙腈体积分数保持为20%,5~10min内流动相乙腈体积分数从20%增至100%,10~15min内流动相乙腈体积分数从100%回至20%)。采用该条件检测得到的茶皂素峰型对称,保留时间合适,杂峰和茶皂素峰分离度较高。

关键词: 高效液相色谱法, 茶籽粕, 茶皂素, 检测

Abstract: In this work, tea saponin was purified by reversed-phase ODS-A column chromatography from degreased coldpressed tea seed meal for use as a reference standard in the quantitative determination of tea saponin by HPLC and identified by UV absorption spectrometry, FTIR and mass spectrometry. HPLC separation was achieved with reversed-phase ODS column and acetonitrile-water as eluent (an initial acetonitrile concentration of 20% held for 5 min, then increased to 100% in 5 min, and finally decreased back to the initial value) at a flow rate of 1.0 mL/min in gradient mode. UV detection was carried out at 215 nm. Under these conditions, symmetric peaks, desired retention time and good separation between tea saponin and impurity were observed.

Key words: high performance liquid chromatography (HPLC), cold-pressed tea seed meal, tea saponin, detection

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