食品科学 ›› 2013, Vol. 34 ›› Issue (11): 36-40.doi: 10.7506/spkx1002-6630-201311009

• 基础研究 • 上一篇    下一篇

人工蛹虫草的化学成分分析

陈 策,图力古尔*,包海鹰   

  1. 吉林农业大学 食药用菌教育部工程研究中心,吉林 长春 130118
  • 收稿日期:2012-01-10 修回日期:2013-04-28 出版日期:2013-06-15 发布日期:2013-06-03
  • 通讯作者: 图力古尔 E-mail:475742561@qq.com

Chemical Composition Analysis of Cultured Cordyceps militaris

CHEN Ce,BAU Tolgor*,BAO Hai-ying   

  1. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,
    Jilin Agricultural University, Changchun 130118, China
  • Received:2012-01-10 Revised:2013-04-28 Online:2013-06-15 Published:2013-06-03
  • Contact: BAU Tolgor E-mail:475742561@qq.com

摘要:

采用硅胶柱色谱法、薄层色谱法、紫外-可见吸收光谱分析法、核磁共振法、液质联用(HPLC-MS/MS)的多反应监测(MRM)等多种分离与鉴定方法,从人工培养蛹虫草的石油醚、氯仿和甲醇层提取物中分离鉴定出8个化合物,并通过GC-MS技术对人工蛹虫草石油醚提取物进行分析,得出其脂肪酸以亚油酸和反油酸为主,含量分别为41.949%、27.696%。分离得到的8个化合物结构分别被鉴定为:玉米黄素、二氢胆甾醇油酸酯、1-油酰基-2-亚油酸-3-棕榈酸甘油、4,6,8(14),22(23)-四烯-3-酮-麦角甾烷、β-谷甾醇、麦角甾醇过氧化物、啤酒甾醇、甘露醇,其中玉米黄素、二氢胆甾醇油酸酯、1-油酰基-2-亚油酸-3-棕榈酸甘油均是首次从蛹虫草中分离得到。

关键词: 玉米黄素, 二氢胆甾醇油酸酯, 1-油酰基-2-亚油酸-3-棕榈酸甘油, 蛹虫草

Abstract:

Eight compounds were isolated from cultured Cordyceps militaris by silica gel column chromatography and
recrystallization. Their structures were identified as zeaxanthin, β-cholestanyl oleate, 1-oleoyl-2-linoleoyl-3-palmitoylglycerol,
4,6,8(14),22(23)-tetraen-3-one-ergostane, β-sitosterol, ergosterol peroxide, cerevisterol and mannitol by thin layer
chromatography (TLC), HPLC-MS/MS, NMR and EI-MS. This study is the first report that zeaxanthin, β-cholestanyl oleate,
and 1-oleoyl-2-linoleoyl-3-palmitoyl glycerol were isolated from Cordyceps militaris. linoleic acid The major fatty acids of
petroleum ether extract of cultured Cordyceps militaris were linoleic acid and elaidic acid, acounting for 41.949% and 27.696%,
respectively, as analyzed by GC-MS.

Key words: zeaxanthin;&beta, -cholestanyl oleate;1-oleoyl-2-linoleoyl-3-palmitoylglycerol;Cordyceps militaris

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