Abstract: The international adopted nutrient value assessment method was applied to the overall assessment of the protein nutrient value of the submerged cultivated mycelium and fruit bodies of Boletus edulis. The mycelium protein content of Boletus edulis is 28.40% and the fruit body is 25.10%, respectively. The first and second limiting amino acids of Boletus edulis mycelium are the sulfur-containing amino acids ( methionine and cystine ) and lysine, and the first and second limiting amino acids of the fruiting bodies are sulfur-containing amino acids and isoleucine.The amino acid score (AAS), chemical score (CS), essential amino acid index (EAAI), biological value (BV), nutritional index (NI) and score of ratio coefficient of amino acid (SRCAA) of the submerged cultivated mycelium protein of Boletus edulis are 31.43, 39.5, 44.55, 36.86, 12.65 and 24.47, compared with corresponding values for fruit bodies are 71.43, 67.02, 63.86, 57.91, 16.03 and 68.55, respectively, higher than that of mycelium protein of Boletus edulis.