食品科学 ›› 2013, Vol. 34 ›› Issue (8): 94-99.doi: 10.7506/spkx1002-6630-201308019

• 工艺技术 • 上一篇    下一篇

正交试验优化胶原多肽螯合钙的制备工艺

彭巧云,沈菊泉,魏东芝,马志英,沈亚领   

  1. 1.华东理工大学生物工程学院,生物反应器工程国家重点实验室,上海 200237;2.上海市食品研究所,上海 200235
  • 收稿日期:2012-01-12 修回日期:2013-02-27 出版日期:2013-04-25 发布日期:2013-05-07
  • 通讯作者: 彭巧云 E-mail:pengqiaoyun12@126.com
  • 基金资助:
    上海市科学技术委员会应用技术开发专项资金

Optimization of Preparation of Collagen Polypeptide Chelated Calcium by Orthogonal Array Design

PENG Qiao-yun,SHEN Ju-quan,WEI Dong-zhi,MA Zhi-ying,SHEN Ya-ling   

  1. 1. State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, Shanghai 200237, China;2. Shanghai Food Research Institute, Shanghai 200235, China
  • Received:2012-01-12 Revised:2013-02-27 Online:2013-04-25 Published:2013-05-07

摘要: 以胶原多肽及氯化钙为原料制备胶原多肽螯合钙并确定其最佳制备工艺,结果表明:胶原多肽螯合钙最适的螯合条件为钙元素和胶原多肽的质量比为13:100、pH6.0、时间30min、温度30℃。在该最佳螯合条件下得到的胶原多肽螯合钙的螯合率达到77.7mg/g。傅里叶变换红外光谱分析结果表明胶原多肽螯合钙是一种新的物质,后期的动物实验也证明了胶原多肽螯合钙具有增加骨密度的功能。

关键词: 胶原多肽, 螯合率, 骨钙, 骨密度

Abstract: This work reports the application of orthogonal array design to explore the optimum chelating conditions of food-grade calciumchloride with collagen polypeptide. The optimal chelating conditions were found to be reaction at 30℃ and an initial pH of 6.0 for 30 min with a calcium-to-collagen polypeptide mass ratio of 13:100. Under the optimized conditions, the calcium-chelating efficiency of collagen polypeptides was 77.7 mg/g. FTIR analysis demonstrated that we successfully obtained collagen polypeptide chelated calcium as a new material. Moreover, the chelated calcium was found to increase bone density in animal studies.

Key words: collagen polypeptide, chelating rate, bone calcium, bone mineral density

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