鹑鸡肠球菌m661的抗氧化、降胆固醇作用

doi:10.7506/spkx1002-6630-201307033

食品科学 ›› 2013, Vol. 34 ›› Issue (7): 157-161.doi: 10.7506/spkx1002-6630-201307033

鹑鸡肠球菌m661的抗氧化、降胆固醇作用

刘长建，刘秋，姜波，闫建芳，孙天竹

大连民族学院生命科学学院，辽宁大连 116600

收稿日期:2012-02-09 修回日期:2013-03-01 出版日期:2013-04-15 发布日期:2013-03-20
通讯作者: 刘长建 E-mail:lcj@dlnu.edu.cn
基金资助:
国家自然科学基金面上项目(31070005)

Antioxidant and Cholesterol-reducing Properties of Enterococcus gallinarum m661

LIU Chang-jian，LIU Qiu，JIANG Bo，YAN Jian-fang，SUN Tian-zhu

College of Life Science, Dalian Nationalities University, Dalian 116600, China

Received:2012-02-09 Revised:2013-03-01 Online:2013-04-15 Published:2013-03-20
Contact: Chang-jian LIU E-mail:lcj@dlnu.edu.cn

摘要/Abstract

摘要： 经厌氧培养，从猪胃液中分离得到1株生长良好的肠球菌m661，菌株能产乳酸、革兰氏染色阳性、接触酶阴性。经系统发育分析，结合菌落形态、细胞形态、生化反应实验，确定菌株m661为鹑鸡肠球菌。鹑鸡肠球菌m661具有抗氧化活性，对羟自由基(?OH)、DPPH自由基和超氧阴离子自由基(O2－?)的清除率与细胞浓度呈正相关。在细胞浓度为5×108CFU/mL时，菌株m661对?OH、DPPH自由基和O2－?的清除率分别为1.58%、36.6%和57.1%。菌株m661还具有清除培养基中34.4%的胆固醇、产生89.9mg/L苯乳酸等益生特性。菌株m661可作为益生菌进行开发利用。

关键词: 猪胃液, 肠球菌属, 16S rDNA, 系统发育树, 抗氧化作用

Abstract: An enterococcal strain named as m661 was isolated from porcine gastric juice. Strain m661 was found to be capable of producing lactic acid, Gram-positive and catalase-negative. Based on phylogenetic analysis, cellular and colonial morphology and biochemical test, m661 was identified as Enterococcus gallinarum. This strain had antioxidant potential, and its scavenging activity against hydroxyl, DPPH and superoxide anion radicals was in a cell density-dependent manner. At a cell density of 5 × 108 CFU/mL, the scavenging rates of stain m661 against hydroxyl, DPPH, and superoxide anion radicals were 1.58%, 36.6% and 57.1%, respectively. In addition, m661 was found to reduce cholesterol by 34.4% in the culture medium and produce 89.9 mg/L phenyllactic acid, suggesting excellent health-benefiting effects. The results of this study demonstrate that strain m661 has great potential to be developed as a new probiotic resource.

Key words: porcine gastric juice, Enterococcus, 16S rDNA, phylogenetic tree, antioxidant effect

中图分类号:

TS201.3

刘长建，刘秋，姜波，闫建芳，孙天竹. 鹑鸡肠球菌m661的抗氧化、降胆固醇作用[J]. 食品科学, 2013, 34(7): 157-161.

LIU Chang-jian，LIU Qiu，JIANG Bo，YAN Jian-fang，SUN Tian-zhu. Antioxidant and Cholesterol-reducing Properties of Enterococcus gallinarum m661[J]. FOOD SCIENCE, 2013, 34(7): 157-161.

参考文献

[1] RAHKILA R, JOHANSSON P, SADE E, et al. Identification of enterococci from broiler products and a broiler processing plant and description of Enterococcus viikkiensis sp. nov.[J]. Appl Environ Microbiol, 2011, 77(4): 1196-1203.
[2] JENNES W, DICKS LM, VERWOERD DJ. Enterocin 012, a bacteriocin produced by Enterococcus gallinarum isolated from the intestinal tract of ostrich[J]. J Appl Microbiol, 2000, 88(2): 349-357.
[3] SUN Peng, WANG Jiaqi, JIANG Yanmei, et al. Effects of Enterococcus faecium (SF68) on immune function in mice[J]. Food Chemistry, 2010, 123(1): 63-68.
[4] PEREIRA DI, GIBSON GR. Cholesterol assimilation by lactic acid bacteria and Bifidobacteria isolated from the human gut[J]. Appl Environ Microbiol, 2002, 68(9): 4689-4693.
[5] HALLIWELL B, CHIRICO S. Lipid peroxidation: its mechanism, measurement, and significance[J]. Am J Clin Nutr, 1993, 57(5suppl): 715S-725S.
[6] KIM JE, KIM JY, LEE KW, et al. Cancer chemopreventive effects of lactic acid bacteria[J]. J Microbiol Biotecnol, 2007, 17(8): 1227-1235.
[7] LIN MY, YEN CL. Antioxidative ability of lactic acid bacteria[J]. J Agric Food Chem, 1999, 47(4): 1460-1466.
[8] 刘天祎, 潘道东. 抗氧化活性乳酸菌的筛选[J]. 食品科学, 2011, 32,(19): 125-129.
[9] 王曦, 罗霞, 许晓燕, 等. 不同乳酸菌菌株抗氧化能力的比较研究[J]. 食品科学, 2010, 31(9): 197-201.
[10] SONGISEPP E, KULLISAAR T, HUTT P, et al. A new probiotic cheese with antioxidative and antimicrobial activity[J]. J Dairy Sci, 2004, 87(7): 2017-2023.
[11] 刘长建, 徐洪涛, 权春善, 等. 乳酸菌素生产菌的分离与鉴定[J]. 食品与发酵工业，2005, 31(7): 26-30.
[12] 凌代文. 乳酸菌分类鉴定及实验方法[M]. 北京：中国轻工业出版社，1998: 117-128.
[13] ORSINI M, ROMANO-SPICA V. A microwave-based method for nucleic acid isolation from environmental samples[J]. Lett Appl Microbiol, 2001, 33(1): 17-20.
[14] 徐平, 李文均, 徐丽华, 等. 微波法快速提取放线菌基因组DNA[J]. 微生物学通报，2003，30(4): 82-84.
[15] 王玉华, 王立梅, 冯印. 鼠李糖乳杆菌菌株LR12和LR76的抗氧化性研究[J]. 食品科学，2009，30(19): 177-180.
[16] 刘长建, 姜波, 刘亮, 等. 枸杞子多糖提取工艺优化及体外抗氧化活性研究[J]. 时珍国医国药，2009，20(3) : 661-663.
[17] JIANG Bo, ZHANG Hongyan, LIU Changjian, et al. Extraction of water-soluble polysaccharide and the antioxidant activity from Ginkgo biloba leaves[J]. Med Chem Res, 2010, 19(3) : 262-270.
[18] EUZEBY JP. List of bacterial names with standing in nomenclature: a folder available on the internet[J]. Int J Syst Bacteriol, 1997, 47(2): 590-592. [2012-01-17]. http://www.bacterio.cict.fr/e/enterococcus.html.
[19] 刘长建，姜波，安晓雯，等. 菠菜中降胆固醇乳酸菌的筛选及鉴定[J]. 食品与生物技术学报，2009，29(6)：937-940.
[20] ARMAFORTE E, CARRI S, FERRI G, et al. High-performance liquid chromatography determination of phenyllactic acid in MRS broth[J]. J Chromatogr A, 2006, 1131(1-2): 281–284.
[21] BRIDGE PD, SNEATH PHA. Streptococcus gallinarum sp. nov. and Streptococcus oralis sp. nov.[J]. Int J Syst Bacteriol, 1982, 32(4): 410-415.
[22] COLLINS MD, JONES D, FARROW JAE, et al. Enterococcus avium nom. rev., comb. nov.; E.casseliflavus nom. rev., comb. nov.; E.durans nom. rev., comb. nov.; E.gallinarum comb. nov.; and E.malodoratus sp. Nov.[J]. Int J Syst Bacteriol, 1984, 34(2): 220-223.
[23] TERAHARA M, KURAMA S, TAKEMOTO N. Prevention by lactic acid bacteria of the oxidation of human LDL[J]. Biosci Biotechnol Biochem, 2001, 65(8): 1864-1868.
[24] 王逸群，钟秀容. 水稻内生固氮细菌的生化特性及其对烟草和玉米的侵染[J]. 云南植物研究，2008, 30 (2): 211-220.
[25] RABAEY K, BOON N, SICILIANO S D, et al. Biofuel cells select for microbial consortia that self-mediate electron transfer[J]. Appl Environ Microbiol, 2004, 70(9) : 5373-5382.
[26] JURKOVIC D, KRIZKOVA L, SOJKA M, et al. Molecular identification and diversity of enterococci isolated from Slovak Bryndza cheese[J]. J Gen Appl Microbiol, 2006, 52: 329-337.

鹑鸡肠球菌m661的抗氧化、降胆固醇作用

Antioxidant and Cholesterol-reducing Properties of Enterococcus gallinarum m661

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