与特定乳酸菌共培养对植物乳杆菌KLDS1.0391细菌素合成的诱导作用

doi:10.7506/spkx1002-6630-201307038

食品科学 ›› 2013, Vol. 34 ›› Issue (7): 180-184.doi: 10.7506/spkx1002-6630-201307038

与特定乳酸菌共培养对植物乳杆菌KLDS1.0391细菌素合成的诱导作用

满丽莉1,2，孟祥晨1,*，赵日红1

1.东北农业大学乳品科学教育部重点实验室，黑龙江哈尔滨 150030； 2.黑龙江农业经济职业学院食品工程学院，黑龙江牡丹江 157041

收稿日期:2011-12-27 修回日期:2013-03-08 出版日期:2013-04-15 发布日期:2013-03-20
通讯作者: 孟祥晨 E-mail:xchmeng@163.com
基金资助:
教育部“创新团队发展计划”项目(IRT0959)；哈尔滨市科技创新人才专项(2011RFLXN017)

Induction of Bacteriocin Synthesis in Lactobacillus plantarum KLDS1.0391 by Some Lactic Acid Bacteria Strains

MAN Li-li1,2，MENG Xiang-chen1,*，ZHAO Ri-hong1

1. Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China； 2. College of Food Engineering, Heilongjiang Agricultural Economy Vocational College, Mudanjiang 157041, China

Received:2011-12-27 Revised:2013-03-08 Online:2013-04-15 Published:2013-03-20
Contact: MENG Xiang-Chen E-mail:xchmeng@163.com

摘要/Abstract

摘要： 植物乳杆菌KLDS1.0391与76株乳酸菌分别共培养后，测定培养物的抑菌活性和活菌数，判断与乳酸菌共培养对植物乳杆菌细菌素合成的影响及细菌素合成与菌体密度的关系。结果表明：植物乳杆菌KLDS1.0706、KLDS4.0315、KLDS4.0351、罗伊氏乳杆菌KLDS1.0736这4株菌与植物乳杆菌KLDS1.0391共培养后，抑菌活性显著增加(P＜0.01)。与罗伊氏乳杆菌共培养过程中细菌素的抑菌活性与植物乳杆菌KLDS1.0391的细胞密度呈现明显的正相关性，并且发现只有活的诱导菌与植物乳杆菌KLDS1.0391共培养才能够诱导细菌素的合成。

关键词: 植物乳杆菌, 细菌素, 共培养, 群体感应

Abstract: The aim of this study was to determine the effects of 76 lactic acid bacteria strains on bacteriocin production by L. plantarum and the relationship between bacteriocin production and cell number. Lactobacillus plantarum KLDS1.0391 was co-cultured with one of 76 lactic acid bacteria strains for the determination of antimicrobial activity and live cell populations. Significantly (P ＜ 0.01) increased antimicrobial activity in the supernatants was obtained by the co-culture of Lactobacillus plantarum KLDS1.0391 with L. plantarum KLDS1.0706, L. plantarum KLDS4.0315, L. plantarum KLDS4.0351 or L. reuteri KLDS1.0736. There was a significant positive correlation between the cell density of Lactobacillus plantarum KLDS1.0391 and the antimicrobial activity of the supernatant of co-culture with L. reuteri KLDS1.0736. We also found that bacteriocin production was induced only when L. plantarum KLDS1.0391 was co-cultured with living cells of inducing strains.

Key words: Lactobacillus plantarum, bacteriocin, co-culture, quorum sensing

中图分类号:

TS252.1

满丽莉1,2，孟祥晨1,*，赵日红1. 与特定乳酸菌共培养对植物乳杆菌KLDS1.0391细菌素合成的诱导作用[J]. 食品科学, 2013, 34(7): 180-184.

MAN Li-li1,2，MENG Xiang-chen1,*，ZHAO Ri-hong1. Induction of Bacteriocin Synthesis in Lactobacillus plantarum KLDS1.0391 by Some Lactic Acid Bacteria Strains[J]. FOOD SCIENCE, 2013, 34(7): 180-184.

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与特定乳酸菌共培养对植物乳杆菌KLDS1.0391细菌素合成的诱导作用

Induction of Bacteriocin Synthesis in Lactobacillus plantarum KLDS1.0391 by Some Lactic Acid Bacteria Strains

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