食品科学 ›› 2013, Vol. 34 ›› Issue (5): 246-250.doi: 10.7506/spkx1002-6630-201305051

• 营养卫生 • 上一篇    下一篇

矢车菊素-3-葡萄糖苷对骨骼肌细胞脂蛋白脂肪酶活性的影响

卫晓怡1,白 晨1,*,唐立伟1,陆 红2,张靖伟1   

  1. 1.上海商学院旅游与食品学院,上海 200235;2.复旦大学生命科学学院,上海 200433
  • 收稿日期:2012-02-07 修回日期:2013-01-15 出版日期:2013-03-15 发布日期:2013-04-16
  • 通讯作者: 白 晨 E-mail:weixy@sbs.edu.cn
  • 基金资助:
    上海市教育委员会科研创新基金

Cyanidin-3-O-β-glucoside Induces Lipoprotein Lipase Activity Following the Activation of AMP-Activated Protein Kinase in Skeletal Muscle Cells

WEI Xiao-yi1,BAI Chen1,*,TANG Li-wei1,LU Hong2,ZHANG Jing-wei1   

  1. 1. Department of Tourism and Food Science, Shanghai Business School, Shanghai 200235, China; 2. School of Life Science, Fudan University, Shanghai 200433, China
  • Received:2012-02-07 Revised:2013-01-15 Online:2013-03-15 Published:2013-04-16
  • Contact: BAI Chen E-mail:weixy@sbs.edu.cn

摘要: 富含矢车菊素-3-葡萄糖苷(Cy-3-g)的花色苷提取物可抑制肥胖,但其作用机制不明。骨骼肌细胞脂蛋白脂肪酶(LPL)活性与肥胖相关,LPL使骨骼肌中的β-脂肪酸氧化增强,从而加速清除体内循环的甘油三酯(TG),抑制TG流向脂肪组织积聚而导致肥胖。本实验建立稳定的骨骼肌细胞分离培养方法,通过花色苷Cy-3-g干预骨骼肌细胞,研究Cy-3-g对细胞LPL活性的影响及其作用机制。结果表明:Cy-3-g通过激活一磷酸腺苷激活的蛋白激酶(AMPK)上调骨骼肌细胞LPL活性,提示Cy-3-g具有潜在的调节机体脂代谢作用,与含Cy-3-g的花色苷提取物的肥胖抑制作用密切相关。

关键词: 矢车菊素-3-葡萄糖苷, 骨骼肌细胞, 脂蛋白脂肪酶, 一磷酸腺苷激活的蛋白激酶

Abstract: Cyanidin-3-O-β-glucoside (Cy-3-g)-rich foods have been reported to inhibit the onset of obesity, but the underlying mechanism has remained unclear. The induction of LPL activity in skeletal muscle will facilitate to the hydrolysis of triglyceride (TG) by β-oxidation, which can result in the decrease of TG store in adipose and the inhibition of obesity. In the present study, the isolation and culture method for skeletal muscle cells was established. The aim of our present study was to explore the effect of Cy-3-g on LPL activity in the skeletal muscle cells isolated from rats. The results demonstrated that Cy-3-g induced the activation of LPL in skeletal muscle cells due to the activation of pAMPK, suggesting that Cy-3-g may have a potential role against obesity through the regulation of lipid metabolism.

Key words: cyanidin-3-O-β-glucoside, skeletal muscle cells, lipoprotein lipase, AMP-activated protein kinase

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