大蒜的生物富硒作用及其硒的形态分析

食品科学 ›› 2012, Vol. 33 ›› Issue (17): 1-5.

• 基础研究 • 下一篇

大蒜的生物富硒作用及其硒的形态分析

方勇1，罗佩竹1，胡勇2，马宁1，杨文建1，辛志宏2，赵立艳2，胡秋辉1,2,*

1.南京财经大学食品科学与工程学院 2.南京农业大学食品科技学院

收稿日期:2012-04-12 修回日期:2012-08-01 出版日期:2012-09-15 发布日期:2012-11-09
通讯作者: 罗佩竹 E-mail:luopeizhu@163.com
基金资助:
公益性行业（农业）科研专项经费

Bioaccumulation and Speciation Analysis of Selenium in Garlic (Allium sativum L.)

Received:2012-04-12 Revised:2012-08-01 Online:2012-09-15 Published:2012-11-09

摘要/Abstract

摘要： 通过大蒜叶面喷施硒肥的田间试验，分析大蒜头和大蒜茎叶中硒含量，采用水提、酸提和酶提3种方法提取大蒜中的硒的形态，利用高效液相色谱联用电感耦合等离子体质谱仪(HPLC-ICP/MS)方法测定普通大蒜和富硒大蒜中的硒形态。结果表明：通过叶面喷施硒肥，大蒜蒜头硒含量从0.047μg/g显著提高到0.200～0.834μg/g (P＜0.05)，大蒜茎叶硒含量从0.043μg/g显著提高到0.291～0.962μg/g(P＜0.05)。通过比较的3种硒形态提取方法，证明酶法提取率最高，为较优方法。普通大蒜和富硒大蒜中的主要硒形态为MeSeCys、SeCys2和SeⅣ。与普通大蒜相比，通过叶面喷施硒肥后，大蒜头中主要转化的有机硒形态为MeSeCys，占总提取硒的81.2%，并进一步通过电喷雾质谱(ESI-MS)鉴定MeSeCys的结构，但酸提液中仍存在未知硒峰，需进一步鉴定。

关键词: 大蒜, 硒, 化学形态, ICP-MS

Abstract: The bioaccumulation and chemical species of selenium in garlic (Allium sativum L.) were investigated. Total selenium content in garlic bulb and stem were determined. The selenium speciation of garlic was analyzed by water extraction, acid extraction or enzymatic extraction followed by HPLC-ICP/MS. The results showed that total selenium content in garlic was significantly increased from 0.047μg/g to 0.200—0.834μg/g (P＜0.05), and total selenium content in stem with 0.043μg/g was significantly increased to 0.291— 0.962μg/g (P＜0.05) by foliar application of Se fertilizer. Compared with water and acid extraction, enzymatic extraction was selected for the extraction of Se species as the preferable method. Subsequently, the major selenospecies in regular garlic and Se-enriched garlic were identified as MeSeCys, SeCys2 and SeⅣ. Compared with regular garlic, the major transformed organic selenospecies in Se-enriched garlic by foliar application was MeSeCys, which represented nearly 81.2% of total selenium in garlic extract. Moreover, MeSeCys from Se-enriched garlic was identified by ESI-MS. However, an unknown selenospecies in acid extract needs to be clarified in future study.

Key words: garlic, selelnium, chemical speciation, ICP-MS

方勇1，罗佩竹1，胡勇2，马宁1，杨文建1，辛志宏2，赵立艳2，胡秋辉1,2,*. 大蒜的生物富硒作用及其硒的形态分析[J]. 食品科学, 2012, 33(17): 1-5.

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大蒜的生物富硒作用及其硒的形态分析

Bioaccumulation and Speciation Analysis of Selenium in Garlic (Allium sativum L.)

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