3种HPLC检测器测定单甘酯含量的方法比较

doi:10.7506/spkx1002-6630-201308033

食品科学 ›› 2013, Vol. 34 ›› Issue (8): 161-163.doi: 10.7506/spkx1002-6630-201308033

3种HPLC检测器测定单甘酯含量的方法比较

方敏，赵谋明，赵强忠

华南理工大学轻工与食品学院，广东广州 510640

收稿日期:2012-03-09 修回日期:2013-03-18 出版日期:2013-04-25 发布日期:2013-05-07
通讯作者: 方敏 E-mail:380306102@qq.com
基金资助:
国家自然科学基金项目;粤港关键领域重点突破项目

Comparison of Three Detectors Used for HPLC Determination of Monoglycerides

FANG Min，ZHAO Mou-ming，ZHAO Qiang-zhong

College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China

Received:2012-03-09 Revised:2013-03-18 Online:2013-04-25 Published:2013-05-07
Contact: Min FANG E-mail:380306102@qq.com

摘要/Abstract

摘要： 比较高效液相色谱-紫外检测法(HPLC-UV)、高效液相色谱-示差折光检测法(HPLC-RI)、高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定乳浊液中单甘酯的含量。结果表明：UV法检测单甘酯，杂质干扰严重，样品信号不强，灵敏度低；RI法只有在较高的质量浓度范围内才有较好的线性关系，不适合用于检测较低质量浓度的单甘酯；ELSD法检测单甘酯含量，单甘酯质量浓度在0.040～5.000mg/mL范围内有良好的线性关系，R2为0.9998，样品回收率达到92.94%，相对标准偏差为2.24%，变异系数为2.41%。HPLC-ELSD法灵敏，准确，重复性好，可用于乳浊液中单甘酯含量的测定。

关键词: 单甘酯, 含量测定, 高效液相色谱-紫外检测, 高效液相色谱-示差折光检测法, 高效液相色谱-蒸发光散射检测

Abstract: High performance liquid chromatography-ultraviolet detector(HPLC-UV), high performance liquid chromatography-differential refractive index detector(HPLC-RI) and high performance liquid chromatography- evaporative light scattering detector(HPLC-ELSD) were compared to measure the content of monoglycerides in emulsions.When using UV detector, the result was seriously influenced by impurities, the signal of the samples was weak and the sensitivity was low. RI detector could had a good linear relationship only in the higher concentration range, so it was not suitable for measuring low content of monoglycerides. When using ELSD detector, the linear range of monoglycerides were between 0.040~5.000 mg/mL. The correlation coefficient of ELSD was 0.9998 , the recovery rate of monoglycerides reached 92.94%, the standard deviations was 2.24 and the variation coefficients was 2.41%. HPLC-ELSD method was sensitive, accurate and reproducible and may therefore be applicable for the determination of monoglycerides in emulsions .

Key words: monoglyceride, content determination, HPLC-UV, HPLC-RI, HPLC-ELSD

中图分类号:

TS252.7

方敏，赵谋明，赵强忠. 3种HPLC检测器测定单甘酯含量的方法比较[J]. 食品科学, 2013, 34(8): 161-163.

FANG Min，ZHAO Mou-ming，ZHAO Qiang-zhong. Comparison of Three Detectors Used for HPLC Determination of Monoglycerides[J]. FOOD SCIENCE, 2013, 34(8): 161-163.

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3种HPLC检测器测定单甘酯含量的方法比较

Comparison of Three Detectors Used for HPLC Determination of Monoglycerides

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