PCR-ELISA法检测食品中空肠弯曲菌

doi:10.7506/spkx1002-6630-201310054

食品科学 ›› 2013, Vol. 34 ›› Issue (10): 246-249.doi: 10.7506/spkx1002-6630-201310054

PCR-ELISA法检测食品中空肠弯曲菌

史艳宇，刘金华，薛力刚，邴炜，华蕾，赵立群，周亮，刘晓晖，王潇，张庆波，冷喜芳

1.吉林省产品质量监督检验院，吉林长春 130022；2.吉林出入境检验检疫局，吉林长春 130062；
3.吉林农业大学，吉林长春 130018

收稿日期:2012-02-16 修回日期:2013-04-02 出版日期:2013-05-25 发布日期:2013-05-07
通讯作者: 张庆波 E-mail:zhangqingbo@163.com
基金资助:
吉林省科技厅项目

Rapid Detection of Campylobacter jejuni in Foods by PCR-ELISA

SHI Yan-yu，LIU Jin-hua，XUE Li-gang，BING Wei，HUA Lei，ZHAO Li-qun，ZHOU Liang，LIU Xiao-hui，WANG Xiao，ZHANG Qing-bo，LENG Xi-fang

1. Jilin Product Quality Supervision Inspection, Changchun 130022, China；2. Jilin Entry-Exit Inspection and Quarant Bureau, Changchun 130062, China；3. Jilin Agricultural University, Changchun 130018, China

Received:2012-02-16 Revised:2013-04-02 Online:2013-05-25 Published:2013-05-07

摘要/Abstract

摘要：

针对空肠弯曲菌16S rRNA基因应用Primer Express 3.0软件设计特异性引物和探针，核酸探针5’端标记生物素，将聚合酶链式反应(PCR)、核酸液杂交以及酶联显色技术结合，对检测条件进行优化，建立食品中空肠弯曲菌的聚合酶链式反应-酶联免疫吸附分析法(PCR-ELISA)。该方法特异性强、灵敏度高(比PCR高10倍)，可以快速、准确检测食品中污染的空肠弯曲菌。该方法可应用于临床诊断、食品卫生监控及出口食品的病原微生物检测等各个领域。

关键词: 空肠弯曲菌, 聚合酶链式反应-酶联免疫吸附分析法, 检测

Abstract:

A PCR-ELISA method was developed to detect Campylobacter jejuni contamination in foods through the combined use of PCR, nucleic acid hybrid reaction and enzyme coloration. A forward PCR primer and a probe labeled with biotin at the 5’ end was designed from Campylobacter jejuni 16S rRNA gene sequence by using Primer Express Software 3.0. The testing conditions were optimized and developed for PCR-ELISA detection of Campylobacter jejuni in foods. The PCRELISA method revealed 10 times higher sensitivity than the ordinary PCR method. PCR-ELISA has potential applications in such fields as clinic diagnosis, food hygiene monitoring and pathogen detection in food imports and exports.

Key words: Campylobacter jejuni, polymerase chain reaction-enzyme linked immunosorbent assay, detection

中图分类号:

TS201.25

史艳宇，刘金华，薛力刚，邴炜，华蕾，赵立群，周亮，刘晓晖，王潇，张庆波，冷喜芳. PCR-ELISA法检测食品中空肠弯曲菌[J]. 食品科学, 2013, 34(10): 246-249.

SHI Yan-yu，LIU Jin-hua，XUE Li-gang，BING Wei，HUA Lei，ZHAO Li-qun，ZHOU Liang，LIU Xiao-hui，WANG Xiao，ZHANG Qing-bo，LENG Xi-fang. Rapid Detection of Campylobacter jejuni in Foods by PCR-ELISA[J]. FOOD SCIENCE, 2013, 34(10): 246-249.

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PCR-ELISA法检测食品中空肠弯曲菌

Rapid Detection of Campylobacter jejuni in Foods by PCR-ELISA

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