食品科学 ›› 2013, Vol. 34 ›› Issue (11): 250-253.doi: 10.7506/spkx1002-6630-201311054

• 营养卫生 • 上一篇    下一篇

四川部分地区猪肉产业链中沙门氏菌的分离及其鉴定

侯小刚,刘书亮,韩新锋,吴聪明   

  1. 1.四川农业大学食品学院,四川 雅安 625014;2.中国农业大学动物医学院,北京 100193
  • 收稿日期:2012-03-02 修回日期:2013-05-03 出版日期:2013-06-15 发布日期:2013-06-03
  • 通讯作者: 刘书亮 E-mail:lsliang999@163.com

Isolation and Identification of Salmonella from Pork Industry Chains in Partial Areas of Sichuan Province

HOU Xiao-gang,LIU Shu-liang,HAN Xin-feng,WU Cong-ming   

  1. 1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China;
    2. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
  • Received:2012-03-02 Revised:2013-05-03 Online:2013-06-15 Published:2013-06-03
  • Contact: Liu Shu-liang E-mail:lsliang999@163.com

摘要:

研究四川猪肉产业链中沙门氏菌的污染和传播情况。自2010年5月至2011年5月从四川部分地区的大型养猪场、下游生猪屠宰场和肉类市场采样829份,采用沙门氏菌科玛嘉显色培养基等分离筛选疑似沙门氏菌,并应用针对沙门氏菌invA和hut基因的二重PCR方法鉴定,再对沙门氏菌分离株进行血清型分析。结果表明:分离鉴定出沙门氏菌共112株;养殖场生猪、屠宰场猪肉和销售市场猪肉中沙门氏菌污染率分别为16.40%、10.71%和10.00%;所检出沙门氏菌共有11种血清型,以德尔卑(S.derby)、鼠伤寒(S.typhimurium)、波茨坦(S.potsdam)、吉韦(S.give)等沙门氏菌为主,优势血清型沙门氏菌呈现由养猪场向生猪屠宰场和肉类市场传播的趋势,需要加强卫生监督。

关键词: 沙门氏菌, 猪肉产业链, 分离鉴定, 血清型

Abstract:

To investigate the contamination and propagation status of Salmonella in pork industry chains from Sichuan
province, and to provide a basis for food safety. CHROMagar was used for isolating Salmonella from 829 samples collected
from large-scale pig farms, downstream slaughterhouses and sale markets in Sichuan during May 2010 to May 2011.These
strains were identified by duplex PCR amplification of the invA and hut genes. Finally, serotyping of these Salmonella
isolates was performed. The results showed that 112 strains of Salmonella were identified from the above samples. Of the
samples from pig farms, slaughterhouses and sale markets, 16.40%, 10.71% and 10.00% were found to be positive for
Salmonella, respectively. A total of 11 Salmonella serotypes were found, and the dominant serotypes were S. derby,
S. typhimurium, S. potsdam and S. give. These dominant serotypes tended to spread from slaugherhouses to sale markets. As
a consequence, the hygine supervision needs to be strengthened.

Key words: Salmonella, pork industry chain, isolation and identification, serotype