食品科学 ›› 2012, Vol. 33 ›› Issue (15): 99-102.

• 基础研究 • 上一篇    下一篇

银合欢种子多糖微结构的原子力显微镜观察

杨永利1,郭守军1,叶文斌2,潘显辉2,林月娟3,林海雄1   

  1. 1. 韩山师范学院
    2.西北师范大学生命科学学院 3.汕头大学分析测试中心
  • 收稿日期:2011-06-25 修回日期:2012-06-24 出版日期:2012-08-15 发布日期:2012-09-07
  • 通讯作者: 杨永利 E-mail:rose_kally@yahoo.com.cn
  • 基金资助:

    猪屎豆种子胶的结构及其降血糖作用研究;银合欢种子多糖的结构研究

Microstructural Observation of Leucaena glaucal Seed Polysaccharides under Atomic Force Microscope

  • Received:2011-06-25 Revised:2012-06-24 Online:2012-08-15 Published:2012-09-07
  • Contact: Yong-Li YANG E-mail:rose_kally@yahoo.com.cn

摘要: 目的:通过对银合欢种子多糖的形貌观察,研究其微结构。方法:采用热水浸提法提取银合欢种子多糖,通过改变溶液的质量浓度、云母基底表面的化学修饰等不同制样方式,用原子力显微镜(AFM)可视化不同样品制备条件形成的聚集体和非聚集体银合欢半乳甘露聚糖结构形貌。结果:样品在不同制备条件下得到膜状、颗粒状的聚集体结构和非聚集体单糖分子结构。结论:银合欢种子多糖的单个糖分子呈线形螺旋状,并具有短的分枝结构,分子链间互相缠绕,而形成网格状。

关键词: 原子力显微镜, 银合欢多糖, 微结构

Abstract: Objective: The microstructure of Leucaena glaucal seed polysaccharides was studied based on morphological observation under atomic force microscope (AFM). Methods: Polysaccharides from were extracted by hot water extraction. The structure and morphology of aggregates and non-aggregates from Leucaena glaucal seed polysaccharides formed through varying polysaccharide concentration or chemically modifying the surface of mica substrates were visualized under AFM. Results: Membranous, granular aggregates structure and non-aggregate structure of a single polysaccharide molecule could be observed in the samples prepared at various conditions. Conclusion: A single Leucaena glaucal seeds polysaccharide molecule was linear spiral, and had short branch structure and entangled molecular chains to form a reticulation structure.

Key words: atomic force microscope, Leucaena glaucal seed polysaccharide, microstructure

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