嗜酸乳杆菌对β-乳球蛋白过敏诱发Thl/Th2细胞平衡的影响

食品科学 ›› 2012, Vol. 33 ›› Issue (15): 279-282.

嗜酸乳杆菌对β-乳球蛋白过敏诱发Thl/Th2细胞平衡的影响

李艾黎1，孟祥晨1,*，徐渐2，邵红1，马冬雪1

1.东北农业大学乳品科学教育部重点实验室 2.东北农业大学食品学院

收稿日期:2011-06-24 修回日期:2012-06-18 出版日期:2012-08-15 发布日期:2012-09-07
通讯作者: 孟祥晨 E-mail:xchmeng@163.com
基金资助:
黑龙江省教育厅科学技术研究项目资助

Effect of Lactobacillus acidophilus on β-Lactoglobulin Induced Mouse Th1/Th2 Cell Imbalance

Received:2011-06-24 Revised:2012-06-18 Online:2012-08-15 Published:2012-09-07
Contact: [中]孟祥晨 [英]MENG Xiang-Chen E-mail:xchmeng@163.com

摘要/Abstract

摘要： 目的：观察嗜酸乳杆菌对牛乳β-乳球蛋白(BLG)致敏小鼠Th1/Th2细胞平衡及血清抗体水平的影响，以研究其缓解过敏反应的作用。方法：用牛乳BLG和弗氏佐剂的混合液腹腔注射诱发BALB/c小鼠致敏，建立动物过敏模型。将实验动物随机分为空白组、过敏组与不同剂量的嗜酸乳杆菌组。采用ELISA法测定各组小鼠血清总IgE、BLG特异性IgE和总IgG含量。体外分离培养各组小鼠脾淋巴细胞，采用ELISA法检测细胞上清液中Th1型细胞因子(IL-12、IFN-γ)和Th2型细胞因子(IL-4)的水平。结果：中、高剂量嗜酸乳杆菌组小鼠的IFN-γ/IL-4比值(代表Th1/Th2细胞平衡)显著高于过敏组(P＜0.05)；而其血清中的总IgE、BLG特异性IgE和总IgG水平显著低于过敏组(P＜0.05)，与空白组相比无差异性(P＞0.05)。结论：嗜酸乳杆菌干预可改善小鼠的BLG过敏症状，其作用可能与促进Th1占优势的Th1/Th2细胞平衡，阻断IgE及IgG分泌相关。

关键词: 嗜酸乳杆菌, β-乳球蛋白, 牛乳过敏

Abstract: Objective: To investigate the effects of Lactobacillus acidophilus on the Th1/Th2 cell balance and antibody production in bovine β-lactoglobulin (BLG)-sensitized mice and evaluate its efficacy to relieve consequent allergic reactions. Methods: BALB/c mice were intraperitoneally injected with a mixture of milk BLG and Freund,s adjuvant to create BLG-sensitized mouse models. The mice were randomly divided into blank group, allergic group and different dose of Lactobacillus acidophilus group. The total IgE, BLG-specific IgE and total IgG contents in the serum of mice were measured by ELISA. Splenic lymphocytes were isolated and cultured in vitro to detect the levels of Thl type cytokine (IL-12, IFN-γ) and Th2 type cytokine (IL-4) in the supernatant by ELISA. Results: The IFN-γ/IL-4 ratio, which represents the Th1/Th2 cell balance, increased significantly in both middle- and high-dose Lactobacillus acidophilus groups compared to the allergic group (P＜0.05). At the same time, their serum levels of total BLG-IgE and total IgG decreased significantly (P＜0.05), but showed no significant difference compared to the blank control (P＞0.05). Conclusion: Intragastric administration of Lactobacillus acidophilus may be effective in preventing and alleviating BLG allergic symptoms by restoring the Th1/Th2 cell balance to Th1 dominance and by inhibiting IgE and IgG production.

Key words: Lactobacillus acidophilus, β-lactoglobulin, milk allergy

中图分类号:

R392

李艾黎1，孟祥晨1,*，徐渐2，邵红1，马冬雪1. 嗜酸乳杆菌对β-乳球蛋白过敏诱发Thl/Th2细胞平衡的影响[J]. 食品科学, 2012, 33(15): 279-282.

参考文献

[1]Carlo C , Francesco B, Barbara B, Luigi C, et al.Cow[J].Italian Journal of Pediatrics,2010,36(5):1816-1824
[2]Pasquale M, Giuseppe M, Maria L P.Food allergy in gastroenterologic diseases: Review of literature[J].World J Gastroenterol,2006,12(48):7744-7752
[3] 王小卉,杨毅,王莹,于晓璐.婴儿肠道菌群的形成及其与食物过敏的关系[J].[J].实用儿科临床杂志,2004,19(9):756-758
[4] Romagnani S. The increased prevalence of allergy and the hygiene hypothesis: missing immune deviation, reduced immune suppression, or both? [J] .[J].Immunology.,2004,112(3):352-363
[5] Flinterman A E, Knol E F, van Ieperen-van Dijk A G, et al. Probiotics have a different immunomodulatory potential in vitro versus ex vivo upon oral administration in children with food allergy[J].[J].Allergy Immunol .,2007, ,143(3):237-244
[6]Akiko T,Shinpei T, Shigeru F, et al. .Lactobacillus Acidophilus Strain L-92 Regulates the Production of Th1 Cytokine as well as Th2 Cytokines[J].Allergology International,2007,56(3):293-301
[7]Yumi I, Noriyuki I, Xiao JZ.Suppressive effects of Bifidobacterium breve strain M-16V on T-Helper Type 2 immune responses in a murine model[J].Biol Pharm Bull,2009,32(4):760-763
[8]王子龙, 林召丰, 黄霞, 王志耕.两种分离β-乳球蛋白方法的比较[J].食品工业科技,2008,l 29(12):153-154
[9]Segawa S, Nakakita Y, Takata Y, Wakita Y, Kaneko T, Kaneda H, Watari J, Yasui H.Effect of oral administration of heat-killed Lactobacillus brevis SBC8803 on total and ovalbumin-specific immunoglobulin E production through the improvement of Th1/Th2 balance[J].International Journal of Food Microbiology,2008,121 (1):1-10
[10]Fujiwara D, Inoue S, Wakabayashi H, Fujii T.The anti-allergic effects of lactic acid bacteria are strain dependent and mediated by effects on both Th1/ Th2 cytokine expression and balance[J].International Archives of Allergy and Immunology,2004,135(3):205-215
[11] Karine A P, Christophe C, Herve B, et al. Evaluation of a high IgE-responder mouse model of allergy to bovine b-lactoglobulin BLG: development of sandwich immunoassays for total and allergen-specific IgE, IgG1and IgG2a in BLG-sensitized mice.[J].J. Immunol.Methods.,2000,235(2):21-32
[12]余叶红, 郭本恒, 吴正钧,王荫榆.干酪乳杆菌LC2W细胞壁组分对RAW2647巨噬细胞吞噬活性的影响[J].中国微生态学杂志,2009,21 (1):23-29
[13]Catharina J, Maria T L, Rob C A, Annika S.Elevated levels of IgG and IgG4 to Malassezia allergens in atopic eczema patients with IgE reactivity to Malassezia[ J].[J].Int Arch Allergy Immunol,2004,135(2):93-100

[1]	黄俊逸，王凤娜，吴香，刘珊，李聪，李新福，徐宝才. 复合发酵剂的筛选及其对发酵香肠加工过程中品质的影响[J]. 食品科学, 2020, 41(24): 95-101.
[2]	张秋月，王刚，陈坤朋，潘道东，曾小群，吴振，郭宇星. 嗜酸乳杆菌在初始弱酸碱条件下对HT-29细胞免疫因子的调节作用[J]. 食品科学, 2020, 41(19): 191-197.
[3]	张娜娜，刘洋，俞漪，赵渝，窦同海，徐琼，段文锋，翁史昱. 乳酸菌饮料中嗜酸乳杆菌的实时荧光定量PCR检测方法[J]. 食品科学, 2019, 40(8): 27-32.
[4]	丛艳君，李晔，刘家琦，陈澍，于晓凤，李林峰. 牛乳α-乳白蛋白免疫球蛋白G线性表位的关键氨基酸识别[J]. 食品科学, 2018, 39(7): 195-200.
[5]	吴京，王文文，熊蓉露，吴振，潘道东，曾小群，郭宇星. 嗜酸乳杆菌ATCC4356分选酶A基因克隆、表达及功能分析[J]. 食品科学, 2018, 39(4): 76-81.
[6]	王泳，贾彦，任效东，明珠，江岩，赵培，庞广昌，阎亚丽，陈庆森. 嗜酸乳杆菌胞外蛋白调控MAPK和PI3K-AKT信号途径关键蛋白活化水平[J]. 食品科学, 2017, 38(3): 155-163.
[7]	丛艳君，陈澍，李晔，于晓凤，李林峰. 牛乳α-乳白蛋白IgE线性表位的关键氨基酸识别[J]. 食品科学, 2017, 38(11): 1-5.
[8]	贾彦，李毛毛，陈蕊，江岩，明珠，赵培，庞广昌，阎亚丽，陈庆森. 杜仲水提液对嗜酸乳杆菌生长及发酵产物的影响[J]. 食品科学, 2016, 37(23): 147-153.
[9]	谢秀玲1,2,3，李欣1,3，陈红兵1,2,*. β-乳球蛋白的聚合及特性研究进展[J]. 食品科学, 2015, 36(9): 230-234.
[10]	赵瑞香，段改丽，杨天佑，孙俊良，牛生洋，王莹. 嗜酸乳杆菌细菌素Lactobacillin XH2抑制大肠杆菌作用机理的探讨[J]. 食品科学, 2015, 36(3): 75-79.
[11]	李晓明，孔阳辉，吕丽爽. 糖基化对β-乳球蛋白结构的影响[J]. 食品科学, 2015, 36(15): 75-80.
[12]	吴振1，潘道东1,2,*，郭宇星1，曾小群2，孙杨赢2. 嗜酸乳杆菌胞壁肽聚糖对小鼠肠黏膜的免疫调控[J]. 食品科学, 2015, 36(13): 207-210.
[13]	赵瑞香，李刚，牛生洋，杨大光，路四海，段改丽，李银娜. 嗜酸乳杆菌胆盐水解酶的分离纯化及酶学性质[J]. 食品科学, 2014, 35(5): 165-168.
[14]	袁水林1,2，熊鼎1,2，陈红兵1,3，高金燕2，李欣1,2,*. 间接竞争ELISA法检测牛乳中β-乳球蛋白含量的准确性评价[J]. 食品科学, 2014, 35(18): 100-104.
[15]	钟俊桢，涂越，刘伟，刘成梅*. 动态高压微射流协同糖基化对β-乳球蛋白乳化性和结构的影响[J]. 食品科学, 2014, 35(1): 7-11.