基于β-葡萄糖苷酶活力的蜂蜜真伪鉴别研究

食品科学 ›› 2012, Vol. 33 ›› Issue (16): 150-153.

基于β-葡萄糖苷酶活力的蜂蜜真伪鉴别研究

张金连，张翠平，郑火青，易松强，胡福良

浙江大学动物科学学院

收稿日期:2011-07-13 修回日期:2012-07-04 出版日期:2012-08-25 发布日期:2012-09-07
通讯作者: 胡福良 E-mail:flhu@zju.edu.cn
基金资助:
国家蜂产业技术体系专项

Discrimination between Authentic and Fake Honey Based on β-Glucosidase Activity

Received:2011-07-13 Revised:2012-07-04 Online:2012-08-25 Published:2012-09-07
Contact: HU Fu-Liang E-mail:flhu@zju.edu.cn

摘要/Abstract

摘要： 提出以β-葡萄糖苷酶活力为指标的蜂蜜真伪鉴别新方法，采用分光光度法分析不同来源蜂蜜中的β-葡萄糖苷酶活力，研究不同储存条件下蜂蜜中β-葡萄糖苷酶活力的变化规律，探讨掺入不同比例大米高果糖浆的蜂蜜中以β-葡萄糖苷酶活力的变化规律。结果表明：不同来源蜂蜜中均能检测出β-葡萄糖苷酶活力，而大米高果糖浆中检测不到该酶活力；不同浓度油菜蜜分别在－18、4、25(室温)、30℃条件下储存11个月，随着储存时间的延长，蜂蜜中β-葡萄糖苷酶活力变化较小；随着油菜蜜中大米高果糖浆的掺假比例增大，β-葡萄糖苷酶活力呈线性下降。因此，β-葡萄糖苷酶活力是检测蜂蜜是否掺假的一个有效定性指标。

关键词: 蜂蜜, β-葡萄糖苷酶, 真伪, 鉴别

Abstract: A new method using β-glucosidase activity was proposed to detect honey adulteration. The activities of β-glucosidase in honey from different plants, different storage conditions, and different proportions of high-fructose rice syrup were determined. The results showed that β-glucosidase activity was detectable in honey samples from different plants; however, no β-glucosidase activity was detected in honey adulterated with high-fructose rice syrup. β-glucosidase activity in rape honey showed little change during 11 months of storage at －18 ℃, 4 ℃, room temperature or 30 ℃. With increasing high-fructose rice syrup adulteration, β-glucosidase activity revealed a linear decline. In conclusion, β-glucosidase activity can be considered as an effective indicator for the identification of honey adulteration.

Key words: honey, β-glucosidase, adulteration, identification

中图分类号:

S896.1

张金连，张翠平，郑火青，易松强，胡福良. 基于β-葡萄糖苷酶活力的蜂蜜真伪鉴别研究[J]. 食品科学, 2012, 33(16): 150-153.

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