小麦肽对大鼠氮代谢以及胃肠黏膜结构和功能的影响

doi:10.7506/spkx1002-6630-201305055

食品科学 ›› 2013, Vol. 34 ›› Issue (5): 264-269.doi: 10.7506/spkx1002-6630-201305055

小麦肽对大鼠氮代谢以及胃肠黏膜结构和功能的影响

潘兴昌1，印虹2，谷瑞增1，徐亚光1，蔡木易1，孙桂菊2

1.中国食品发酵工业研究院，北京 100028；2.东南大学公共卫生学院，江苏南京 210009

收稿日期:2012-03-09 修回日期:2013-01-28 出版日期:2013-03-15 发布日期:2013-04-16
通讯作者: 潘兴昌 E-mail:nutripan@163.com

Effect of Wheat Peptide on the Nitrogen Metabolism and Gastrointestinal Mucosal Structure of Rats

PAN Xing-chang1，YIN Hong2，GU Rui-zeng1，XU Ya-guang1，CAI Mu-yi1，SUN Gui-ju2

1. China National Research Institute of Food and Fermentation Industries, Beijing 100028, China； 2. School of Public Health, Southeast University, Nanjing 210009, China

Received:2012-03-09 Revised:2013-01-28 Online:2013-03-15 Published:2013-04-16
Contact: PAN Xing-chang E-mail:nutripan@163.com

摘要/Abstract

摘要： 目的：观察灌胃小麦肽对于大鼠氮代谢、胃肠形态和小肠黏膜酶活的影响。方法：SD大鼠50只，随机分为空白对照组及低、中、高剂量小麦肽组(灌胃剂量分别为20、100、500mg/(kg·d))和小麦蛋白组20mg/(kg·d))，每天灌胃1次，连续灌胃30d。分别于第10、20、30天用代谢笼采集大鼠24h粪、尿样品。灌胃30d后断颈处死大鼠，取血清、胃和小肠黏膜，观察氮代谢指标、血清生化指标、胃肠内表面扫描电镜、小肠黏膜酶活等的变化。结果：灌胃30d后，低剂量小麦肽能显著提高大鼠蛋白质消化率、氮沉积、净蛋白质利用率(P＜0.05)；低、中剂量小麦肽均能显著增加大鼠蛋白质生物学价值(P＜0.05)；低、中、高剂量小麦肽均能显著增加大鼠血清总蛋白含量(P＜0.05)；低、中剂量小麦肽大鼠胃肠道上皮细胞较空白对照组饱满、连接紧密，排列整齐有序；低、中、高剂量小麦肽均能显著上调大鼠小肠黏膜氨基肽酶活力(P＜0.05)；中、高剂量小麦肽均能上调大鼠小肠黏膜Na+-K+-ATP酶活力(P＜0.05)。结论：一定剂量的小麦肽能提高大鼠对于蛋白质的吸收与利用，其可能的途径是：促进胃肠道上皮细胞的生长；小肠黏膜氨基肽酶、Na+-K+-ATP酶活力的上调。

关键词: 小麦肽, 氮代谢, 胃小肠形态, 氨基肽酶, Na+-K+-ATP酶

Abstract: Purpose: The present study was aimed to investigate the potential effect of the wheat peptide on the nitrogen metabolism, gastrointestinal morphology, and enzyme activity of small intestine mucosa in rats. Methods: 50 SD rats were randomly divided into control group, low, medium and high doses of the wheat peptide group and wheat gluten group. The animals were daily administered with water, wheat peptide or wheat protein through ig route for continuous 30 days. The feces and urine samples of rats in 24 hours were collected using metabolic cages on 10th, 20th, 30th day. On 30th days, rats were sacrificed and serum, stomach and small intestine mucosa were harvested to investigate the changes of the indicators of nitrogen metabolism, serum biochemical parameters, surface scanning electron microscopy of gastrointestinal and enzyme activity of small intestinal mucosa. Results: Following 30 days of treatment, low dose of wheat peptide can significantly increase the rat protein digestibility, nitrogen deposition, net protein utilization and the protein biological values were also enhanced by low- and mid-dose of wheat peptide administration. Wheat peptide at all given doses was able to increase the serum total protein content. The gastrointestinal tract epithelial cells of rat following feeding low and medium dose of wheat peptide were more fully lined, tightly connected, neat and orderly than those of control group. Low, middle and high dose wheat peptide could also increase the aminopeptidase enzyme activity of intestinal mucous membrane and high doses of wheat peptide was able to enhance the Na+-K+-ATPase activity of intestinal mucous membrane. Conclusion: a certain dose of wheat peptide can improve the protein absorption and utilization of rats and the mechanisms might be related to its benefits in promoting the growth of epithelial cells of the gastrointestinal tract and its capacity to enhance the aminopeptidase and Na+-K+-ATPase activities of small intestinal mucosa.

Key words: wheat peptide, nitrogen metabolism, gaster & small intestine-morph, aminopeptidase, Na+-K+-ATPase

中图分类号:

S858

潘兴昌1，印虹2，谷瑞增1，徐亚光1，蔡木易1，孙桂菊2. 小麦肽对大鼠氮代谢以及胃肠黏膜结构和功能的影响[J]. 食品科学, 2013, 34(5): 264-269.

PAN Xing-chang1，YIN Hong2，GU Rui-zeng1，XU Ya-guang1，CAI Mu-yi1，SUN Gui-ju2. Effect of Wheat Peptide on the Nitrogen Metabolism and Gastrointestinal Mucosal Structure of Rats[J]. FOOD SCIENCE, 2013, 34(5): 264-269.

参考文献

[ ] Huebner FR, Lieberman KW, Rubino RP, et al. Demonstration of high opioid-like activity in isolated peptides from wheat gluten hydrolysates[J]. Peptides,1984,5(6):1139-1147
[ ]Shin-ichi F, Masaaki Y. Opioid peptides derived from wheat gluten: their isolation and characterization[J]. FEBS Letters,1992,296(1):107-111
[ ]Calzuola I, Giavarini F, Sassi P, et al. Short acidic peptides isolated from wheat sprout chromatin and involved in the control of cell proliferation Characterization by infrared spectroscopy and mass spectrometry[J]. Peptides, 2005, 26:2074-2085
[ ]Motoi H, Kodama T. Isolation and characterization of angiotensin I-converting enzyme inhibitory peptides from wheat gliadin hydrolysate[J]. Nahrung-food,2003,47(5): 354-358
[ ]Jia JQ, Ma H, Zhao W,et al. The use of ultrasound for enzymatic preparation of ACE-inhibitory peptides from wheat germ protein[J]. Food Chemistry,2010,119:336-342
[ ]孔祥珍,周惠明,钱海峰.小麦面筋蛋白短肽的镇痛作用研究[J].食品与生物技术学报,2007,26(2):26-29
[ ]代卉,乐国伟,孙进,等.小麦肽对受环磷酰胺免疫抑制小鼠的免疫调节及抗氧化功能[J].生物工程学报,2009,25(4):549-553
[ ]叶丽萍,龚四堂.正常大鼠小肠黏膜纹状缘氨基肽酶活性的研究[J].广东医学,2008,29(5):746-747
[ ]Li ZT,Li DF,Qiao SY.Effects of soybean agglutinin on nitrogen metabolism and on characteristics of intestinal tissues and pancreas in rats[J]. Arch Anim Nutr,2003,57(5):369-380
[ ]Auricchio S, De RG, De VM,et al. Toxicity mechanisms of wheat and other cereals in celiac disease and related enteropaties[J]. J Ped Gastr Nutr,1985,4,923-930
[ ]Boze JJ, Moennoz D, Vuichoud J, et al. Protein hydrolysate vs free amino acid-based diets on the nutritional recovery of the starved rat[J]. European Journal of Nutrition, 2000, 39(6):237-243
[ ]Stewart JS. A study of proximal and distal intestinal structure and absorptive function in idiopathic steatorrhea[J]. Quart J Med,1976,36:425
[ ]王恬,傅永明,吕俊龙,等.小肽营养素对断奶仔猪生产性能及小肠发育的影响[J].畜牧与兽医,2003,35(6):4-7
[ ]Mane S, Gade W, Jamdar S.Purification and characterization of proline aminopeptidase from chicken intestine[J].Process Biochemistry,2011,46(6):1384-1389.
[ ]邹思湘.动物生物化学[M].第四版.北京:中国农业出版社,2005:102-103

[1]	李彦华，杨芸，徐卫红，周鑫斌，王卫中，迟荪琳，李桃，张春来，赵婉伊，秦余丽，王正银，谢德体. 不同小白菜品种硝酸盐含量、氮代谢关键酶活性及NRT1表达和亚细胞定位[J]. 食品科学, 2018, 39(7): 78-84.
[2]	陈永勤，冯勃，徐卫红，杨芸，迟荪琳，陈序根，王正银，谢德体. 小白菜硝酸盐含量与光照度及氮代谢关键酶的相关性[J]. 食品科学, 2016, 37(13): 183-188.
[3]	迟荪琳，杨芸，徐卫红，陈序根，陈永勤，谢文文，熊仕娟，王正银，谢德体. 小白菜硝酸盐含量与NO3－/NH4+及氮代谢关键酶的相关性[J]. 食品科学, 2015, 36(23): 70-77.
[4]	郭宇星1，祝倩1，朱坤1，周慧敏1，孙杨赢1,2，曾小群1,2，潘道东1,2,*. 瑞士乳杆菌X-脯氨酰-二肽酰基-氨基肽酶的分离纯化及酶学性质[J]. 食品科学, 2013, 34(17): 156-160.
[5]	印虹马畅韩东宁张源淑. 乳源β- 酪啡肽-7 对高血糖大鼠小肠黏膜氧化应激的影响[J]. 食品科学, 2011, 32(3 ): 208-211.
[6]	郭宇星1，潘道东1,2,*，田俊英1. 瑞士乳杆菌产氨肽酶和X-脯氨酰-二肽酰基-氨基肽酶发酵条件的优化[J]. 食品科学, 2009, 30(7): 148-153.
[7]	周瑞华, 唐永梅, 周小芸. 蜂王幼虫蛋白质的营养评价[J]. 食品科学, 1991, 12(6): 12-15.

小麦肽对大鼠氮代谢以及胃肠黏膜结构和功能的影响

Effect of Wheat Peptide on the Nitrogen Metabolism and Gastrointestinal Mucosal Structure of Rats

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价