食品有害物质与DNA相互作用的体外研究方法

doi:10.7506/spkx1002-6630-201307064

摘要/Abstract

摘要： 食品有害物质与DNA相互作用模式的体外研究方法，包括电化学法、紫外-可见吸收光谱法、荧光光谱法、凝胶电泳法和黏度法等；食品有害物质与DNA相互作用的定量方法，包括32P后标记法、免疫分析法、免疫毛细血管电泳-激光诱导荧光法和液相色谱-串联质谱法等。本文在查阅近10a相关文献的基础上，对体外研究DNA相互作用的检测技术和分析方法进行综述，以期为食品有害物质与DNA的相互作用研究提供一定的参考。

关键词: DNA, 相互作用, 体外研究方法

Abstract: The research methods such as electrochemical, UV-visible, fluorescence, SCGE and viscosity methods for the interaction between harmful substances in foods and DNA have been reviewed. A number of techniques and methods such as 32P-postlabelling, immunoassay, immuno-CE/LIF and LC/MS have also been developed for the quantitative detection of interaction between harmful substances in foods and DNA. Based on the research of recently 10 years of related literature, the detection technique and analytic methods regarding in vitro investigation of DNA interaction have been also briefly discussed in this article, which will provide valuable references for the studies of interaction between food harmful substances and DNA.

Key words: DNA, interaction, study methods in vitro

中图分类号:

TS207.3

关郁芳1，雷丹丹1，谭玉荣1，赵国华1,2,*. 食品有害物质与DNA相互作用的体外研究方法[J]. 食品科学, 2013, 34(7): 302-306.

GUAN Yu-fang1，LEI Dan-dan1，TAN Yu-rong1，ZHAO Guo-hua1,2,*. in vitro Research Methods of Interaction between Harmful Substances in Foods and DNA[J]. FOOD SCIENCE, 2013, 34(7): 302-306.

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