食品科学 ›› 2013, Vol. 34 ›› Issue (13): 162-164.doi: 10.7506/spkx1002-6630-201313035

• 生物工程 • 上一篇    下一篇

不同pH值酿酒酵母分批发酵生产谷胱甘肽数学模型建立

郑丽雪1,2,谢群凡1,王立梅1,2,齐 斌1,*   

  1. 1.常熟理工学院生物与食品工程学院,江苏 常熟 215500;2.苏州市食品生物技术重点实验室,江苏 常熟 215500
  • 收稿日期:2012-03-31 修回日期:2013-05-14 出版日期:2013-07-15 发布日期:2013-06-28
  • 通讯作者: 齐斌 E-mail:qibin65@126.com
  • 基金资助:

    国家自然科学基金面上项目(31171758);2011年度常熟理工学院“青蓝工程”优秀青年骨干教师培养对象项目

Mathematical Modeling of Glutathione Production by Saccharomyces cerevisiae in Batch Fermentation at Different pH

ZHENG Li-xue1,2,XIE Qun-fan1,WANG Li-mei1,2,QI Bin1,*   

  1. 1. College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China;
    2. Suzhou Key Laboratory of Food and Biotechnology, Changshu 215500, China
  • Received:2012-03-31 Revised:2013-05-14 Online:2013-07-15 Published:2013-06-28
  • Contact: QI Bin E-mail:qibin65@126.com

摘要:

以麦芽汁为发酵培养基,考察不同pH值条件下酿酒酵母CS10515-1分批发酵合成谷胱甘肽的影响,对实验 数据进行分析并拟合出数学模型。结果表明:两种pH值条件下高斯拟合对谷胱甘肽产量数据的拟合效果较好,相 关系数(R2)分别为0.99235(pH5.0)、0.98842(pH6.0),而对生物量、基质消耗拟合效果差。另外,当发酵pH值恒定在 5.0时,32h时谷胱甘肽产量达最大,为72mg/L,此时生物量为4.59g/L;当发酵pH值恒定为6.0时,同样也是32h时谷 胱甘肽产量达最大,为91mg/L,此时生物量为4.11g/L。

关键词: 谷胱甘肽, 麦芽汁, 酿酒酵母, 高斯拟合

Abstract:

A mathematical model of glutathione production by Saccharomyces cerevisiae Y518 in batch fermentation at
different pH was established with malt sprout extract as the fermentation medium. The results showed that Gaussian fitting
model was in good agreement with the experimental observations for glutathione production at different pH, with correlation
coefficient (R2) of 0.99235 (pH5.0) and 0.98842 (pH6.0), but not suitable for biomass and substrate consumption prediction.
Glutathione production reached the maximum of 72 mg/L at 32 h yielding a biomass of 4.59 g/L when pH was kept constant
at 5.0. In contrast, maximum glutathione production of 91 mg/L was also obtained at 32 h along with a biomass of 4.11 g/L
when pH was kept constant at 6.0.

Key words: glutathione, malt sprout extract, Saccharomyces cerevisiae, Gaussian fitting

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