响应曲面法优化匀浆提取银杏叶原花青素工艺

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 12-16.

响应曲面法优化匀浆提取银杏叶原花青素工艺

王燕芹1，徐莉1,2,*，曹福亮3，陈天婵1，阎艳红1，雷萌1，陈泳1，唐颖1，蒋国斌1，汪贵斌3

1.南京林业大学理学院 2.南京林业大学现代分析测试中心 3.南京林业大学森林资源与环境学院

收稿日期:2012-05-08 修回日期:2012-10-24 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 徐莉 E-mail:xuliqby@163.com
基金资助:
国家科技支撑计划课题;国家林业局林业公益性行业科研专项;国家林业局林业公益性行业科研专项

Optimization of Homogenization Conditions for the Extraction of Proanthocyanidin from Ginkgo biloba Leaves Using Response Surface Methodology

Received:2012-05-08 Revised:2012-10-24 Online:2012-11-25 Published:2012-11-20

摘要/Abstract

摘要： 采用匀浆法提取银杏叶中的原花青素，在单因素试验的基础上，采用响应曲面法对提取过程中的工艺参数进行了优化。根据Box-Behnken中心组合试验设计原理，考察了提取溶剂、料液比、匀浆转速和匀浆提取时间等因素，以原花青素提取率为响应值进行响应面分析。响应面优化后提取工艺条件为：以无水乙醇为提取溶剂，料液比1:23.8(g/mL)、匀浆时间5.87min、转速10500r/min，在此条件下原花青素的提取率为2.21%。

关键词: 银杏叶, 原花青素, 匀浆提取, 响应面分析

Abstract: This study deals with the optimization of homogenization conditions for the extraction of proanthocyanidin from Ginkgo biloba leaves using response surface methodology. The effects of extraction solvent, solid-to-solvent ratio, and homogenization speed and time on the extraction yield of proanthocyanidin was investigated by one-factor-at-a-time method. Further investigations were carried out to optimize the process parameters through response surface analysis of a mathematical model for the extraction yield of proanthocyanidin as their function built based on a Box-Behnken experimental design. The optimal conditions for extracting proanthocyanidin from Ginkgo biloba leaves were found to be homogenization at a rotary speed of 10500 r/min for 5.87 min with an absolute ethanol-to-Ginkgo biloba leaves of 1:23.8 (g/mL). Under these conditions, the extraction yield of proanthocyanidin was 2.21%.

Key words: Ginkgo biloba leaves, proanthocyanidin, homogenization, extraction, response surface methodology

中图分类号:

王燕芹1，徐?莉1,2,*，曹福亮3，陈天婵1，阎艳红1，雷?萌1，陈?泳1，唐?颖1，蒋国斌1，汪贵斌3 . 响应曲面法优化匀浆提取银杏叶原花青素工艺[J]. 食品科学, 2012, 33(22): 12-16.

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