葡萄酒乳酸菌种内遗传多样性鉴定

食品科学 ›› 2012, Vol. 33 ›› Issue (21): 183-186.

葡萄酒乳酸菌种内遗传多样性鉴定

但霞1，李凯1，朱成龙1，刘树文1,2,*

1.西北农林科技大学葡萄酒学院 2.陕西省葡萄与葡萄酒工程技术研究中心

收稿日期:2012-05-15 修回日期:2012-10-18 出版日期:2012-11-15 发布日期:2012-11-09
通讯作者: 刘树文 E-mail:liushuwen@nwsuaf.edu.cn
基金资助:
现代农业产业技术体系建设专项资金资助项目

Intraspecific Genetic Diversity of Lactic Acid Bacteria from Grape Wine

Received:2012-05-15 Revised:2012-10-18 Online:2012-11-15 Published:2012-11-09

摘要/Abstract

摘要： 为了研究葡萄酒乳酸菌从自然启动苹乳发酵的葡萄酒中分离乳酸菌，并对其进行生化鉴定和16S rRNA序列测定，结果显示分离菌种有酒类酒球菌、马里乳杆菌、植物乳杆菌和短乳杆菌4种乳酸菌。同时利用单酶切扩增片段长度多态性(SE-AFLP)对其进行种内基因多样性鉴定，并绘制种内进化树，结果表明SE-AFLP在乳酸菌分类研究中能够达到菌株水平的区分。

关键词: 葡萄酒, 乳酸菌, SE-AFLP, 种内鉴定

Abstract: Lactic acid bacteria isolated from spontaneously Malolactic fermented grape swine were identified by biochemical identification and 16S rRNA sequencing. The results showed that the strains were Oenococcus oeni, Lactobacillus mali, Lactobacillus plantarum and Lactobacillus brevis. Intraspecific genetic diversity of these strains was further analyzed by single-enzyme amplified fragment length polymorphism (SE-AFLP) and phylogenetic tree was constructed by the method of pair group method with arithmetic Mean (UPGMA). The results showed that SE-AFLP could identify lactic acid bacteria to strain level in lactic acid bacteria classification.

Key words: grape wine, lactic acid bacteria, single-enzyme amplified fragment length polymorphism (SE-AFLP), intraspecific identification

中图分类号:

TS261.1

但?霞1，李?凯1，朱成龙1，刘树文1,2,*. 葡萄酒乳酸菌种内遗传多样性鉴定[J]. 食品科学, 2012, 33(21): 183-186.

参考文献

[1] 张春晖,李华.葡萄酒微生物学[M].西安:陕西人民出版社,2003：113-160.
[2] M. Coton , A. Romano , G. Spano et al. Occurrence of biogenic amine-forming lactic acid bacteria in wine and cider[J]. Food Microbiology , 2010,27:1078-1085.
[3] H.Teixera,M.G.Goncalves,N.Rozes et al. Lactobacillic acid accumulation in the plasma membrane of Oenococcus oeni: A response to Ethanol Stress?[J].Microb Ecol,2002,43:146-153.
[4] P. Garijo , R. López , P. Santamaría et al. Presence of lactic bacteria in the air of a winery during the vinification period[J]. International Journal of Food Microbiology, 2009,136:142–146.
[5] Giovanni M. Giammanco , Caterina Mammina , Cristina Romani. Evaluation of a modified single-enzyme amplified fragment length polymorphism (SE-AFLP) technique for subtyping Salmonella enteric serotype Enteritidis[J]. Research in Microbiology, 2007,158 : 10~17.
[6] 金刚.中国葡萄酒主产区酒酒球菌的鉴定及SE-AFLP分析[D].西北农林科技大学，2010.
[7] A. M. Moreno, M. R. Baccaro, A. J. P. Ferreira. Use of Single-Enzyme Amplified Fragment Length Polymorphism for Typing Pasteurella multocida subsp. multocida Isolates from Pigs[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 1743~1746.
[8] Ayman Gaafar, M. Josebe Unzaga, Ramo′n Cisterna. Evaluation of a Modified Single-Enzyme Amplified-Fragment Length Polymorphism Technique for Fingerprinting and Differentiating of Mycobacterium kansasii Type I Isolates[J].. JOURNAL OF CLINICAL MICROBIOLOGY,2003, 3846~3850
[9] 李西柱,刘树文,王坐京. 酒酒球菌基因组DNA提取及RAPD反应体系的优化[J].酿酒科技, 2009, 4: 46 - 50.
[10] 马妮妮,魏东霞,林瑞庆等.AFLP标记在微生物学上的应用[J].热带医学杂志, 2006, 6(9):1048-1051.
[11] M.S. Cappello, D. Stefani, F. Grieco. Genotyping by Amplified Fragment Length Polymorphism and malate metabolism performances of indigenous Oenococcus oeni strains isolated from Primitivo wine[J]. International Journal of Food Microbiology, 2008,127 :241~245
[12]Nileena Velappana, James L. Snodgrassb, Janetta R. Hakovirta. Rapid identification of pathogenic bacteria by single-enzyme amplified fragment length polymorphism analysis[J]. Diagnostic Microbiology and Infectious Disease, 2001,39 :77~83
[13]Braulio Esteve-Zarzoso a, Núria Hierro a, Albert Mas. A new simplified AFLP method for wine yeast strain typing[J].. LWT - Food Science and Technology 2010,43:1480~1484
[14] 季鹏章，吕才有，梁名志.应用AFLP 技术鉴别不同地区的普洱茶晒青毛茶[J].食品科学, 2010, 31(24)：264~267.
[15] 刘树文，李西柱，王坐京等. 酒酒球菌抗酸相关基因RAPD 标记的筛选[J]. 食品科学, 2010, 31(7)：190-195.